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Determination of antibiotic EC50 using a zero-flow microfluidic chip based growth phenotype assay

Title
Determination of antibiotic EC50 using a zero-flow microfluidic chip based growth phenotype assay
Author
Jong Wook Hong
Keywords
Antibiotic susceptibility; Growth phenotype assay; Half maximal effective concentration (EC50); Logarithmic concentration gradient; Microfluidics
Issue Date
2015-06
Publisher
WILEY-V C H VERLAG GMBH
Citation
BIOTECHNOLOGY JOURNAL, v. 10, NO 11, Page. 1783-1791
Abstract
Current existing assay systems for evaluating antimicrobial activity suffer from several limitations including excess reagent consumption and inaccurate concentration gradient preparation. Recently, microfluidic systems have been developed to provide miniaturized platforms for antimicrobial susceptibility assays. However, some of current microfluidic based assays require continuous flows of reagents or elaborate preparation steps during concentration preparation. In this study, we introduce a novel microfluidic chip based growth phenotype assay that automatically generates a logarithmic concentration gradient and allows observing the growth of pathogenic bacteria under different concentrations of antibiotics in nanoliter batch culture reactors. We chose pathogen bacterium Pseudomonas aeruginosa as a model strain and evaluated the inhibitory effects of gentamicin and ciprofloxacin. We determined the EC50 values and confirmed the validity of the present system by comparing the EC50 values obtained through conventional test tube method. We demonstrated that the EC50 values acquired from present assay are comparable to those obtained from conventional test tube cultures. The potential application of present assay system for investigating combinatorial effects of antibiotics on multidrug resistant pathogenic bacteria is discussed and it can be further used for systematic evaluation of antifungal or antiviral agents.
URI
http://onlinelibrary.wiley.com/doi/10.1002/biot.201500037/abstract;jsessionid=7BFCAEED286FF9ED558870A727BFB77E.f04t02http://hdl.handle.net/20.500.11754/25938
ISSN
1860-6768; 1860-7314
DOI
10.1002/biot.201500037
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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