Feasibility of islet magnetic resonance imaging using ferumoxytol in intraportal islet transplantation

Title
Feasibility of islet magnetic resonance imaging using ferumoxytol in intraportal islet transplantation
Authors
이동윤
Keywords
Islet; Transplantation; Magnetic resonance imaging; Nanoparticle
Issue Date
2015-06
Publisher
ELSEVIER SCI LTD
Citation
BIOMATERIALS, v. 52, Page. 272-280
Abstract
There is a clinical need for an alternative labeling agent for magnetic resonance imaging (MRI) in islet transplantation. We aimed to evaluate the feasibility of islet MRI using ferumoxytol, which is the only clinically-available ultrasmall superparamagnetic iron oxide. We compared islet function and viability of control islets and islets labeled with ferumoxytol and/or a heparin-protamine complex (HPF). Efficacy of ferumoxytol labeling was assessed in both ex vivo and in vivo models. Labeling for 48 h with HPF, but not up to 800 mu g/mL ferumoxytol, deranged ex vivo islet viability and function. The T2* relaxation time was optimal when islets were labeled with 800 mu g/mL of ferumoxytol for 48 h. Prussian blue stain, iron content assay, transmission electron microscopy (TEM) supported internalization of ferumoxytol particles. However, the labeling intensity in the ex vivo MRI of islets labeled with ferumoxytol was much weaker than that of islets labeled with ferucarbotran. In syngeneic intraportal islet transplantation, there was a correlation between the total area of visualized islets and the transplanted islet mass. In conclusion, islet MRI using ferumoxytol was feasible in terms of in vitro and in vivo efficacy and safety. However, the weak labeling efficacy is still a hurdle for the clinical application. (C) 2015 Elsevier Ltd. All rights reserved.
URI
http://www.sciencedirect.com/science/article/pii/S0142961215001799http://hdl.handle.net/20.500.11754/25866
ISSN
0142-9612; 1878-5905
DOI
http://dx.doi.org/10.1016/j.biomaterials.2015.02.055
Appears in Collections:
COLLEGE OF ENGINEERING[S](공과대학) > BIOENGINEERING(생명공학과) > Articles
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