Fast and sensitive detection of an anthrax biomarker using SERS-based solenoid microfluidic sensor

Title
Fast and sensitive detection of an anthrax biomarker using SERS-based solenoid microfluidic sensor
Authors
주재범
Keywords
Anthrax; Surface-enhanced Raman scattering; Microfluidics; Solenoid; Poly-gamma-D-glutamic acid
Issue Date
2015-06
Publisher
ELSEVIER ADVANCED TECHNOLOGY
Citation
BIOSENSORS & BIOELECTRONICS, v. 72, Page. 230-236
Abstract
We report the application of a fully automated surface-enhanced Raman scattering (SERS)-based solenoid-embedded microfluidic device to the quantitative and sensitive detection of anthrax biomarker poly-gamma-D-glutamic acid (PGA) in solution. Analysis is based on the competitive reaction between PGA and PGA-conjugated gold nanoparticles with anti-PGA-immobilized magnetic beads within a microfluidic environment. Magnetic immunocomplexes are trapped by yoke-type solenoids embedded within the device, and their SERS signals were directly measured and analyzed. To improve the acccuracy of measurement process, external standard values for PGA-free serum were also measured through use of a control channel. This additional measurement greatly improves the reliability of the assay by minimizing the influence of extraneous experimental variables. The limit of detection (LOD) of PGA in serum, determined by our SERS-based microfluidic sensor, is estimated to be 100 pg/mL. We believe that the defined method represents a valuable analytical tool for the detection of anthrax-related aqueous samples. (C) 2015 Elsevier B.V. All rights reserved.
URI
http://www.sciencedirect.com/science/article/pii/S0956566315301019http://hdl.handle.net/20.500.11754/25800
ISSN
0956-5663; 1873-4235
DOI
http://dx.doi.org/10.1016/j.bios.2015.05.005
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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