Development of receptor-based inhibitory RNA aptamers for anthrax toxin neutralization

Title
Development of receptor-based inhibitory RNA aptamers for anthrax toxin neutralization
Authors
윤문영
Keywords
Anthrax toxin; ATR; RNA aptamer
Issue Date
2015-06
Publisher
ELSEVIER SCIENCE BV
Citation
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, v. 77, Page. 293-302
Abstract
Anthrax toxin excreted by Bacillus anthracis is the key causative agent of infectious anthrax disease. In the present study, we targeted the binding of PA to the ATR/TEM8 Von Willebrand factor type A (VWA) domain, which we cloned into Escherichia colt and purified to homogeneity under denaturing conditions. To develop an anthrax toxin inhibitor, we selected and identified short single strand RNA aptamers (approximately 30mer) consisting of different sequences of nucleic acids with a high binding affinity in the 100 nanomolar range against the recombinant ATR/TEM8 VWA domain using systematic evolution of ligands by exponential enrichment (SELEX). Five candidate aptamers were further characterized by several techniques including secondary structural analysis. The inhibitor efficiency (IC50) of one of the aptamers toward anthrax toxin was approximately 5 mu M in macrophage RAW 264.7 cells, as determined from cytotoxicity analysis by MU assay. We believe that the candidate aptamers should be useful for blocking the binding of PA to its receptor in order to neutralize anthrax toxin. (C) 2015 Elsevier B.V. All rights reserved.
URI
http://www.sciencedirect.com/science/article/pii/S0141813015001956http://hdl.handle.net/20.500.11754/25742
ISSN
0141-8130; 1879-0003
DOI
http://dx.doi.org/10.1016/j.ijbiomac.2015.03.043
Appears in Collections:
COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > CHEMISTRY(화학과) > Articles
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