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Characterization of a novel endoglucanase from Ganoderma lucidum

Title
Characterization of a novel endoglucanase from Ganoderma lucidum
Author
Klaus Heese
Keywords
Carboxymethyl cellulose; Endoglucanase; Ganoderma lucidum; Fermentation; Wheat bran
Issue Date
2015-06
Publisher
WILEY-BLACKWELL
Citation
JOURNAL OF BASIC MICROBIOLOGY, v. 55, NO 6, Page. 761-771
Abstract
We evaluated the production and characterization of endoglucanase from Ganoderma lucidum using different lignocellulose biomasses. We purified a novel carboxymethyl cellulose (CMC) hydrolyzing endoglucanase from the white-rot fungus G. lucidum when the medium was supplemented with 1% (w/v) wheat bran. Endoglucanase was purified 12.5-fold via ammonium sulfate fractionation, Sephadex G-100, and Q-Sepharose column chromatography with a final yield of 15%. SDS-PAGE analysis revealed that the endoglucanase had a molecular mass of 64.0kDa. The optimal activity of purified endoglucanase was at pH 5.0 and 35 degrees C, though it was stable between pH 4.0-7.0 and temperatures of 30-60 degrees C. The purified enzyme was specific to CMC as a suitable substrate. The metal ions Hg2+, Fe2+, and Cr2+ inhibited enzyme activity, while Ca2+, Mg2+, and Mn2+ enhanced enzyme activity. The endoglucanase showed high activity and stability in the presence of different surfactants and non-polar hydrophobic organic solvents. This endoglucanase is tolerant to high temperature, metal ions, surfactants, and solvents, suggesting that it is appropriate for use in biomass conversion for biofuel production under harsh environmental conditions.
URI
http://onlinelibrary.wiley.com/doi/10.1002/jobm.201400808/fullhttp://hdl.handle.net/20.500.11754/25691
ISSN
0233-111X; 1521-4028
DOI
10.1002/jobm.201400808
Appears in Collections:
GRADUATE SCHOOL OF BIOMEDICAL SCIENCE AND ENGINEERING[S](의생명공학전문대학원) > ETC
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