mRNA 3 '-UTR shortening is a molecular signature of mTORC1 activation

Title
mRNA 3 '-UTR shortening is a molecular signature of mTORC1 activation
Authors
김계성
Keywords
3' UNTRANSLATED REGIONS; ALTERNATIVE POLYADENYLATION; GENE-EXPRESSION; CLEAVAGE; PROTEIN; CELLS; HOMEOSTASIS; MECHANISMS; REVEALS; TSC1
Issue Date
2015-06
Publisher
NATURE PUBLISHING GROUP
Citation
NATURE COMMUNICATIONS, v. 6, Page. 1-9
Abstract
Mammalian target of rapamycin (mTOR) enhances translation from a subset of messenger RNAs containing distinct 5'-untranslated region (UTR) sequence features. Here we identify 3'-UTR shortening of mRNAs as an additional molecular signature of mTOR activation and show that 3'-UTR shortening enhances the translation of specific mRNAs. Using genetic or chemical modulations of mTOR activity in cells or mouse tissues, we show that cellular mTOR activity is crucial for 3'-UTR shortening. Although long 3'-UTR-containing transcripts minimally contribute to translation, 3-'UTR-shortened transcripts efficiently form polysomes in the mTOR-activated cells, leading to increased protein production. Strikingly, selected E2 and E3 components of ubiquitin ligase complexes are enriched by this mechanism, resulting in elevated levels of protein ubiquitination on mTOR activation. Together, these findings identify a previously uncharacterized role for mTOR in the selective regulation of protein synthesis by modulating 3'-UTR length of mRNAs.
URI
http://www.nature.com/articles/ncomms8218http://hdl.handle.net/20.500.11754/25572
ISSN
2041-1723
DOI
http://dx.doi.org/10.1038/ncomms8218
Appears in Collections:
COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
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