175 0

Supplementation of Growth Differentiation Factor-5 Increases Proliferation and Size of Chondrogenic Pellets of Human Umbilical Cord-Derived Perivascular Stem Cells

Title
Supplementation of Growth Differentiation Factor-5 Increases Proliferation and Size of Chondrogenic Pellets of Human Umbilical Cord-Derived Perivascular Stem Cells
Author
김계성
Keywords
Perivascular stem cells; Growth differentiation factor-5; Proliferation; Chondrocytes; AUTOLOGOUS CHONDROCYTE IMPLANTATION; CARTILAGE REPAIR; ARTICULAR CHONDROCYTES; STROMAL CELLS; BONE-MARROW; IN-VITRO; TISSUE; EXPRESSION; CULTURE; GDF-5
Issue Date
2015-06
Publisher
KOREAN TISSUE ENGINEERING REGENERATIVE MEDICINE SOC
Citation
TISSUE ENGINEERING AND REGENERATIVE MEDICINE, v. 12, NO 3, Page. 181-187
Abstract
In recent years, perivascular stem cells (PVCs) have gained increasing attention as a promising source for regenerative medicine due to their greater differentiation potential compared to multipotent mesenchymal stem cells (MSCs). It has been reported that growth differentiation factor-5 (GDF-5) is involved in regulating proliferation and chondrogenic differentiation of MSCs. In this study, we investigated the effect of GDF-5 on the proliferation and chondrogenic differentiation of PVCs isolated from human umbilical cords. The supplementation of PVC cultures with GDF-5 (100 ng/mL) significantly enhanced their proliferative rate and augmented the size of pellets in micromass pellet cultures for chondrogenic induction. Although similar expression levels of chondrogenic-related genes were observed in chondrogenic pellets treated with GDF-5 compared to the pellets without GDF-5 treatment, these results indicate that supplementation of GDF-5 is able to acquire more chondrocytes when starting with equal amount of PVCs. Our study suggests that GDF-5 is an effective agent for the enhancement of PVC proliferation, thereby achieving a higher number of chondrocytes that are applicable in therapeutic doses for cartilage regeneration.
URI
http://link.springer.com/article/10.1007/s13770-015-0113-4http://hdl.handle.net/20.500.11754/25545
ISSN
1738-2696
DOI
10.1007/s13770-015-0113-4
Appears in Collections:
COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE