Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 신흥수 | - |
dc.date.accessioned | 2016-12-13T02:14:07Z | - |
dc.date.available | 2016-12-13T02:14:07Z | - |
dc.date.issued | 2015-05 | - |
dc.identifier.citation | SMALL, v. 11, Page. 2069-2079 | en_US |
dc.identifier.issn | 1613-6810 | - |
dc.identifier.issn | 1613-6829 | - |
dc.identifier.uri | http://onlinelibrary.wiley.com/doi/10.1002/smll.201402933/abstract | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/24822 | - |
dc.description.abstract | Stem cells are poorly permissive to non-viral gene transfection reagents. In this study, we explored the possibility of improving gene delivery into human embryonic (hESC) and mesenchymal (hMSC) stem cells by synergizing the activity of a cell-binding ligand with a polymer that releases nucleic acids in a cytoplasm-responsive manner. A 29 amino acid long peptide, RVG, targeting the nicotinic acetylcholine receptor (nAchR) was identified to bind both hMSC and H9-derived hESC. Conjugating RVG to a redox-sensitive biodegradable dendrimer-type arginine-grafted polymer (PAM-ABP) enabled nanoparticle formation with plasmid DNA without altering the environment-sensitive DNA release property and favorable toxicity profile of the parent polymer. Importantly, RVG-PAM-ABP quantitatively enhanced transfection into both hMSC and hESC compared to commercial transfection reagents like Lipofectamine 2000 and Fugene. similar to 60% and 50% of hMSC and hESC were respectively transfected, and at increased levels on a per cell basis, without affecting pluripotency marker expression. RVG-PAM-ABP is thus a novel bioreducible, biocompatible, non-toxic, synthetic gene delivery system for nAchR-expressing stem cells. Our data also demonstrates that a cell-binding ligand like RVG can cooperate with a gene delivery system like PAM-ABP to enable transfection of poorly-permissive cells. | en_US |
dc.description.sponsorship | J. Beloor and S. Ramakrishna contributed equally to this work. This work was supported by the Connecticut Innovations Stem Cell, State of Connecticut (13-SCA-YALE-38) to P.K. and Korea National Research Foundation Grant (20090079989, 2012R1A6A1029029) to S.K.L. We thank Laura Niklason and Julio J. Mendez of Yale University for the kind gift of hMSC. | en_US |
dc.language.iso | en | en_US |
dc.publisher | WILEY-V C H VERLAG GMBH | en_US |
dc.subject | STABLE TRANSGENE EXPRESSION | en_US |
dc.subject | SIRNA DELIVERY | en_US |
dc.subject | ACETYLCHOLINE-RECEPTOR | en_US |
dc.subject | POLY(DISULFIDE AMINE) | en_US |
dc.subject | EDITING TECHNOLOGIES | en_US |
dc.subject | CHOLINERGIC SYSTEM | en_US |
dc.subject | EFFICIENT | en_US |
dc.subject | THERAPY | en_US |
dc.subject | PROLIFERATION | en_US |
dc.subject | POLYSACCHARIDE | en_US |
dc.title | Effective Gene Delivery into Human Stem Cells with a Cell-Targeting Peptide-Modified Bioreducible Polymer | en_US |
dc.type | Article | en_US |
dc.relation.volume | 11 | - |
dc.identifier.doi | 10.1002/smll.201402933 | - |
dc.relation.page | 2069-2079 | - |
dc.relation.journal | SMALL | - |
dc.contributor.googleauthor | Beloor, Jagadish | - |
dc.contributor.googleauthor | Ramakrishna, Suresh | - |
dc.contributor.googleauthor | Nam, Kihoon | - |
dc.contributor.googleauthor | Choi, Chang Seon | - |
dc.contributor.googleauthor | Kim, Jongkil | - |
dc.contributor.googleauthor | Kim, Sung Hwa | - |
dc.contributor.googleauthor | Cho, Hyong Jin | - |
dc.contributor.googleauthor | Shin, HeungSoo | - |
dc.contributor.googleauthor | Kim, Hyongbum | - |
dc.contributor.googleauthor | Kim, Sung Wan | - |
dc.relation.code | 2015000566 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF ENGINEERING[S] | - |
dc.sector.department | DEPARTMENT OF BIOENGINEERING | - |
dc.identifier.pid | hshin | - |
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