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A novel nanoprobe for the sensitive detection of Francisella tularensis

Title
A novel nanoprobe for the sensitive detection of Francisella tularensis
Author
최종훈
Keywords
Nanoprobes; Francisella tularensis; Apoferritin; Immunoassay; Biosensing
Issue Date
2015-05
Publisher
ELSEVIER SCIENCE BV
Citation
JOURNAL OF HAZARDOUS MATERIALS, v. 298, Page. 188-194
Abstract
Francisella tularensis is a human zoonotic pathogen and the causative agent of tularemia, a severe infectious disease. Given the extreme infectivity of F. tularensis and its potential to be used as a biological warfare agent, a fast and sensitive detection method is highly desirable. Herein, we construct a novel detection platform composed of two units: (I) Magnetic beads conjugated with multiple capturing antibodies against F. tularensis for its simple and rapid separation and (2) Genetically-engineered apoferritin protein constructs conjugated with multiple quantum dots and a detection antibody against F. tularensis for the amplification of signal. We demonstrate a 10-fold increase in the sensitivity relative to traditional lateral flow devices that utilize enzyme-based detection methods. We ultimately envision the use of our novel nanoprobe detection platform in future applications that require the highly-sensitive on-site detection of high-risk pathogens. (C) 2015 Elsevier B.V. All rights reserved.
URI
http://www.sciencedirect.com/science/article/pii/S0304389415004379http://hdl.handle.net/20.500.11754/24466
ISSN
0304-3894; 1873-3336
DOI
10.1016/j.jhazmat.2015.05.041
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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