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dc.contributor.author이양순-
dc.date.accessioned2016-11-18T01:49:18Z-
dc.date.available2016-11-18T01:49:18Z-
dc.date.issued2015-05-
dc.identifier.citationBIOMED RESEARCH INTERNATIONAL, v. 2015, Page. 1-9en_US
dc.identifier.issn2314-6133-
dc.identifier.issn2314-6141-
dc.identifier.urihttps://www.hindawi.com/journals/bmri/2015/535027/-
dc.identifier.urihttp://hdl.handle.net/20.500.11754/24460-
dc.description.abstracta While pulsed-field gel electrophoresis (PFGE) is recognized as the gold standard method for clonality analysis, MALDI-TOF MS has recently been spotlighted as an alternative tool for species identification. Herein, we compared the dendrograms ofmulti-drugresistant (MDR) Acinetobacter baumannii isolates by using MALDI-TOF MS with those by using PFGE. We used direct colony and protein extraction methods for MALDI-TOF MS dendrograms. The isolates with identical PFGE patterns were grouped into different branches in MALDI-TOF MS dendrograms. Among the isolates that were classified as very close isolates in MALDIT-OF MS dendrogram, PFGE band patterns visually showed complete differences. We numeralized similarity among isolates by measuring distance levels. The Spearman rank correlation coefficient values were 0.449 and 0.297 between MALDI-TOF MS dendrogram using direct colony and protein extraction method versus PFGE, respectively. This study is the first paper focusing solely on the dendrogram function of MALDI-TOF MS compared with PFGE. Although MALDI-TOF MS is a promising tool to identify species in a rapid manner, our results showed that MALDI-TOF MS dendrograms could not substitute PFGE for MDR Acinetobacter baumannii clonality analysis.en_US
dc.language.isoenen_US
dc.publisherHINDAWI PUBLISHING CORPORATIONen_US
dc.subjectFIELD GEL-ELECTROPHORESISen_US
dc.subjectMALDI-TOF MSen_US
dc.subjectPSEUDOMONAS-AERUGINOSAen_US
dc.subjectIDENTIFICATIONen_US
dc.subjectOUTBREAKen_US
dc.subjectEPIDEMIOLOGYen_US
dc.subjectIMPROVEMENTen_US
dc.subjectCOMPLEXen_US
dc.titleInsufficient Discriminatory Power of Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry Dendrograms to Determine the Clonality of Multi-Drug-Resistant Acinetobacter baumannii Isolates from an Intensive Care Uniten_US
dc.typeArticleen_US
dc.relation.volume535027-
dc.identifier.doi10.1155/2015/535027-
dc.relation.page1-9-
dc.relation.journalBIOMED RESEARCH INTERNATIONAL-
dc.contributor.googleauthorRim, John Hoon-
dc.contributor.googleauthorLee, Yangsoon-
dc.contributor.googleauthorHong, Sung Kuk-
dc.contributor.googleauthorPark, Yongjung-
dc.contributor.googleauthorKim, MyungSook-
dc.contributor.googleauthorD'Souza, Roshan-
dc.contributor.googleauthorPark, Eun Suk-
dc.contributor.googleauthorYong, Dongeun-
dc.contributor.googleauthorLee, Kyungwon-
dc.relation.code2015012204-
dc.sector.campusS-
dc.sector.daehakCOLLEGE OF MEDICINE[S]-
dc.sector.departmentDEPARTMENT OF MEDICINE-
dc.identifier.pidyangsoon-
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COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
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