SERS-based monitoring of the intracellular pH in endothelial cells: the influence of the extracellular environment and tumour necrosis factor-alpha

Title
SERS-based monitoring of the intracellular pH in endothelial cells: the influence of the extracellular environment and tumour necrosis factor-alpha
Authors
주재범
Keywords
SURFACE-ENHANCED RAMAN; 4-MERCAPTOBENZOIC ACID; SCATTERING; NANOSENSORS; NANOPARTICLES; SILICA; ENDOCYTOSIS; APOPTOSIS; SILVER; PROBE
Issue Date
2015-04
Publisher
ROYAL SOC CHEMISTRY
Citation
ANALYST, v. 140, Page. 2321-2329
Abstract
The intracellular pH plays an important role in various cellular processes. In this work, we describe a method for monitoring of the intracellular pH in endothelial cells by using surface enhanced Raman spectroscopy (SERS) and 4-mercaptobenzoic acid (MBA) anchored to gold nanoparticles as pH-sensitive probes. Using the Raman microimaging technique, we analysed changes in intracellular pH induced by buffers with acid or alkaline pH, as well as in endothelial inflammation induced by tumour necrosis factor-alpha (TNF alpha). The targeted nanosensor enabled spatial pH measurements revealing distinct changes of the intracellular pH in endosomal compartments of the endothelium. Altogether, SERS-based analysis of intracellular pH proves to be a promising technique for a better understanding of intracellular pH regulation in various subcellular compartments.
URI
http://pubs.rsc.org/en/Content/ArticleLanding/2015/AN/C4AN01988A#!divAbstracthttp://hdl.handle.net/20.500.11754/24065
ISSN
0003-2654; 1364-5528
DOI
http://dx.doi.org/10.1039/c4an01988a
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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