Construction of target-specific virus-like particles for the delivery of algicidal compounds to harmful algae

Title
Construction of target-specific virus-like particles for the delivery of algicidal compounds to harmful algae
Authors
진언선
Keywords
DINOFLAGELLATE HETEROCAPSA-CIRCULARISQUAMA;; STRANDED RNA VIRUS; THIAZOLIDINEDIONE DERIVATIVES; CAPSID PROTEIN; RED TIDE; FLOCCULATION; BLOOMS; HCRNAV; CLAY; TD49
Issue Date
2015-04
Publisher
WILEY-BLACKWELL
Citation
ENVIRONMENTAL MICROBIOLOGY, v. 17, Page. 1463-1474
Abstract
Harmful algal blooms (HABs) can lead to substantial socio-economic losses and extensive damage to aquatic ecosystems, drinking water sources and human health. Common algicidal techniques, including ozonation, ultrasonic treatment and dispersion of algae-killing chemicals, are unsatisfactory both economically and ecologically. This study therefore presents a novel alternative strategy for the efficient control of deleterious algae via the use of host-specific virus-like particles (VLPs) combined with chemically synthesized algicidal compounds. The capsid protein of HcRNAV34, a single-stranded RNA virus that infects the toxic dinoflagellate, Heterocapsa circularisquama, was expressed in and purified from Escherichia coli and then self-assembled into VLPsin vitro. Next, the algicidal compound, thiazolidinedione 49 (TD49), was encapsidated into HcRNAV34 VLPs for specific delivery to H. circularisquama. Consequently, HcRNAV34 VLPs demonstrated the same host selectivity as naturally occurring HcRNAV34 virions, while TD49-encapsidated VLPs showed a more potent target-specific algicidal effect than TD49 alone. These results indicate that target-specific VLPs for the delivery of cytotoxic compounds to nuisance algae might provide a safe, environmentally friendly approach for the management of HABs in aquatic ecosystems.
URI
http://onlinelibrary.wiley.com/doi/10.1111/1462-2920.12650/abstract;jsessionid=FF2EAEDB4B21C18C165B7F4F73FA1427.f02t02http://hdl.handle.net/20.500.11754/23910
ISSN
1462-2912; 1462-2920
DOI
http://dx.doi.org/10.1111/1462-2920.12650
Appears in Collections:
COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > LIFE SCIENCE(생명과학과) > Articles
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