One-Pot Enzymatic Conversion of Carbon Dioxide and Utilization for Improved Microbial Growth

Title
One-Pot Enzymatic Conversion of Carbon Dioxide and Utilization for Improved Microbial Growth
Authors
진언선
Keywords
ALGA DUNALIELLA-SALINA; CO2 CAPTURE; ANHYDRASE;; MICROALGAE
Issue Date
2015-04
Publisher
AMER CHEMICAL SOC
Citation
ENVIRONMENTAL SCIENCE & TECHNOLOGY, v. 49, NO 7, Page. 4466-4472
Abstract
We developed a process for one-pot CO2 conversion and utilization based on simple conversion of CO2 to bicarbonate at ambient temperature with no energy input, by using the cross-linking-based composites of carboxylated polyaniline nanofibers (cPANFs) and carbonic anhydrase. Carbonic anhydrase was immobilized on cPANFs via the approach of magnetically separable enzyme precipitate coatings (Mag-EPC), which consists of covalent enzyme attachment, enzyme precipitation, and cross-linking with amine-functionalized magnetic nanoparticles. Mag-EPC showed a half-life of 236 days under shaking, even resistance to 70% ethanol sterilization, and recyclability via facile magnetic separation. For one-pot CO2 conversion and utilization, Mag-EPC was used to accelerate the growth of microalga by supplying bicarbonate from CO2, representing 1.8-fold increase of cell concentration when compared to the control sample. After two repeated uses via simple magnetic separation, the cell concentration with Mag-EPC was maintained as high as the first cycle. This one-pot CO2 conversion and utilization is an alternative as well as complementary process to adsorption-based CO2 capture and storage as an environmentally friendly approach, demanding no energy input based on the effective action of the stabilized enzyme system.
URI
http://pubs.acs.org/doi/abs/10.1021/es505143fhttp://hdl.handle.net/20.500.11754/23776
ISSN
0013-936X; 1520-5851
DOI
http://dx.doi.org/10.1021/es505143f
Appears in Collections:
COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > LIFE SCIENCE(생명과학과) > Articles
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