Rapid Detection of Lactobacillus kefiranofaciens in Kefir Grain and Kefir Milk Using Newly Developed Real-Time PCR

Title
Rapid Detection of Lactobacillus kefiranofaciens in Kefir Grain and Kefir Milk Using Newly Developed Real-Time PCR
Authors
Hyunsook Kim
Keywords
LACTIC-ACID BACTERIA; CULTURE-INDEPENDENT METHODS; TIBET KEFIR; M1
Issue Date
2015-04
Publisher
INT ASSOC FOOD PROTECTION
Citation
JOURNAL OF FOOD PROTECTION, v. 78, NO 4, Page. 855-858
Abstract
Lactobacillus kefiranofaciens is an indicator microorganism for kefir and a key factor in kefir grain formation and kefiran production. We designed a novel real-time PCR primer and probe set, LKF_KU504, for the rapid detection of L. kefiranofaciens. In inclusivity and exclusivity tests, only 14 L. kefiranofaciens strains were positive among 61 microorganisms, indicating 100% sensitivity and specificity. The LKF_KU504 set also differentiated kefir milk from 30 commercial nonkefir yogurts. The levels of L. kefiranofaciens in kefir grain and kefir milk were significantly different, indicating L. kefiranofaciens was more concentrated in kefir grain than in kefir milk
URI
http://search.proquest.com/docview/1668018751?accountid=11283http://hdl.handle.net/20.500.11754/23695
ISSN
0362-028X; 1944-9097
DOI
http://dx.doi.org/10.4315/0362-028X.JFP-14-329
Appears in Collections:
COLLEGE OF HUMAN ECOLOGY[S](생활과학대학) > FOOD & NUTRITION(식품영양학과) > Articles
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