Highly sensitive immunoassay of anti-CCP marker using SERS detection

Title
Highly sensitive immunoassay of anti-CCP marker using SERS detection
Author
주재범
Keywords
surface-enhanced Raman scattering; anti-cyclic citrullinated peptide antibody; rheumatoid arthritis; immunoassay; hollow gold nanosphere; magnetic bead; early diagnosis; blood sample
Issue Date
2015-02
Publisher
SPIE
Citation
SPIE 9523, International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2015, Page. 952301-952308
Abstract
We report a highly sensitive anti-cyclic citrullinated peptide (anti-CCP) detection method for early diagnosis of rheumatoid arthritis (RA) using surface-enhanced Raman scattering (SERS)-based immunoassay. Herein, cyclic citrullinated peptide (CCP)-conjugated magnetic beads and anti-human IgG-conjugated hollow gold nanospheres (HGNs) were used as substrates and SERS nano-tags, respectively. First, its detection sensitivity was evaluated using anti-CCP standard solutions. Then quantitative anti-CCP levels, determined by the SERS-based assay, were compared with those obtained from three commercially available anti-CCP assay kits (Immunoscan CCPlus, ImmunnLisa™ CCP and BioPlex™ 2200) to assess its potential utility as a clinical tool. Finally, clinical samples from 20 RA patients were investigated using them. In the SERS-based assay, the anti-CCP level in human serum was successfully determined by monitoring the characteristic Raman peak intensity of SERS nano-tags. The diagnostic performance of our SERS-based immunoassay for clinical samples shows a good agreement with those measured by three commercial anti-CCP kits. In addition, our SERS-based assay results are more consistent in the low concentration range (0–25 U/mL) than those achieved by the commercial kits. Accordingly, it is estimated that the SERS-based assay is a potentially useful diagnostic tool for early diagnosis of RA. © (2015) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
URI
http://proceedings.spiedigitallibrary.org/proceeding.aspx?articleid=2396906http://hdl.handle.net/20.500.11754/22300
ISSN
0277-786X
DOI
10.1117/12.2189760
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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