Reduced Expression of Osteonectin and Increased Natural Killer Cells May Contribute to the Pathophysiology of Aplastic Anemia

Title
Reduced Expression of Osteonectin and Increased Natural Killer Cells May Contribute to the Pathophysiology of Aplastic Anemia
Authors
이영호
Keywords
aplastic anemia; bone marrow microenvironment; nestin; natural killer cell; MESENCHYMAL STEM-CELLS; HEMATOPOIETIC PROGENITOR CELLS; HUMAN PARATHYROID-HORMONE; EX-VIVO EXPANSION; BONE-MARROW; CORD BLOOD; IN-VITRO; NICHE; OSTEOPONTIN; MICE
Issue Date
2015-02
Publisher
LIPPINCOTT WILLIAMS & WILKINS
Citation
APPLIED IMMUNOHISTOCHEMISTRY & MOLECULAR MORPHOLOGY, v. 23, NO 2, Page. 139-145
Abstract
Normal hematopoiesis involves complex interactions between hematopoietic cells and the bone marrow (BM) microenvironment. The exact causes and mechanisms involved in aplastic anemia (AA) are not known. For better understanding of the pathophysiology of AA, we investigated changes in the hematopoietic stem cell (HSC) compartment and the BM microenvironment in patients with AA by immunohistochemical analysis. A total of 10 AA patients and 10 controls were enrolled. Using BM biopsy specimen, we performed immunohistochemistry for osteopontin, osteonectin, osteocalcin, nestin, stromal-derived factor-1 (SDF-1), lymphocytes, macrophage, and HSCs. Numbers of HSCs and T/B lymphocytes were significantly lower in the AA specimens than the controls, and the AA specimens contained more natural killer cells (CD56(+) cells) (P ˂ 0.01). The 2 groups had similar levels of expression of osteopontin, osteocalcin, nestin, and SDF-1. However, the number of osteonectin(+) cells in the AA specimens was significantly lower than in the control specimens (P ˂ 0.01). Our findings support the hypothesis that defects in the stromal cells contribute to the pathogenesis of AA by damaging HSC niche. Immune-mediated natural killer cells may also play a role in the pathogenesis of AA.
URI
http://hdl.handle.net/20.500.11754/22027http://journals.lww.com/appliedimmunohist/pages/articleviewer.aspx?year=2015&issue=02000&article=00009&type=abstract
ISSN
1541-2016
DOI
http://dx.doi.org/10.1097/PAI.0000000000000023
Appears in Collections:
COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE