Chromosomal cephalosporinase in Enterobacter hormaechei as an ancestor of ACT-1 plasmid-mediated AmpC beta-lactamase

Title
Chromosomal cephalosporinase in Enterobacter hormaechei as an ancestor of ACT-1 plasmid-mediated AmpC beta-lactamase
Author
이양순
Keywords
SP-NOV; RESISTANCE; CLOACAE; GENES; EXPRESSION; ASBURIAE; COMPLEX
Issue Date
2012-01
Publisher
SOC GENERAL MICROBIOLOGY
Citation
JOURNAL OF MEDICAL MICROBIOLOGY, v. 61, Page. 94-100
Abstract
In this study of the diversity of AmpC beta-lactamase in clinical isolates of Enterobacter spp., a strain was found carrying the plasmid-mediated AmpC beta-lactamase ACT-1 gene on its chromosome. The strain was identified as Enterobacter hormaechei using phylogenetic analysis of 16S rRNA and hsp60 genes. In addition, the species was confirmed by DNA DNA hybridization. The genetic environment of the bla(ACT-1) gene was characterized, including the ampR and ampG genes, using a two-step PCR. The amino acid sequences of AmpR at serine 35, arginine 86, glycine 102, aspartic acid 135 and tyrosine 264 were conserved. Measurement of the transcription level of the bla(ACT-1), gene using real-time quantitative PCR showed that it increased 1.98-fold following cefoxitin induction. These results suggest that the plasmid-mediated bla(ACT-1) gene originated from the chromosome of E. hormaechei.
URI
http://jmm.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.032573-0
ISSN
0022-2615
DOI
10.1099/jmm.0.032573-0
Appears in Collections:
COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
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