One step in-situ synthesis of amine functionalized graphene for immunosensing of cardiac marker cTnI

Title
One step in-situ synthesis of amine functionalized graphene for immunosensing of cardiac marker cTnI
Authors
Akash Deep
Keywords
Graphene; 2-Aminobenzyl amine; Biomarker; Cardiac TroponinI; Immunosensor
Issue Date
2015-01
Publisher
Elsevier
Citation
Biosensors and Bioelectronics, v. 66, Page. 129-135
Abstract
2-Aminobenzyl amine (2-ABA) functionalized graphene is proposed for the ultrasensitive immunosensing of Cardiac Troponin I (cTnI). 2-ABA was electrochemically polymerized on the graphene decorated interdigitated electrode to obtain the amine functionalized graphene (f-GN). The f-GN electrode was then modified with monoclonal anti-cTnI antibodies via Schiff reaction based chemistry. Detailed characteristics of the processes involved and the finally developed antibody conjugated f-GN interdigitated electrode have been studied. The above micro-device was used in a drain source configuration for the sensing of cTnI. A wide dynamic linear range of antigen detection (0.01–1 ng/mL) is achieved with the limit of detection of 0.01 ng/mL. The utility of the proposed sensing technique is demonstrated by successfully testing the antigen concentration in spiked serum samples.
URI
http://hdl.handle.net/20.500.11754/21747http://www.sciencedirect.com/science/article/pii/S0956566314008720
ISSN
0956-5663
DOI
http://dx.doi.org/10.1016/j.bios.2014.10.072
Appears in Collections:
COLLEGE OF ENGINEERING[S](공과대학) > ETC
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