Surface-Enhanced Raman Scattering-Based Competitive Immunoassay of Poly-γ-D-Glutamic Acid for Highly Sensitive Detection of Anthrax

Title
Surface-Enhanced Raman Scattering-Based Competitive Immunoassay of Poly-γ-D-Glutamic Acid for Highly Sensitive Detection of Anthrax
Authors
최종훈최종훈
Keywords
Surface-enhanced Raman scattering; Bacillus anthracis; Poly-γ-D-glutamic acid; Competitive immunoassay; Magnetic bead
Issue Date
2015-01
Publisher
KOREAN CHEMICAL SOC
Citation
BULLETIN OF THE KOREAN CHEMICAL SOCIETY, v. 36, NO 3, Page. 822-826
Abstract
Anthrax is a highly acute disease caused by the bacterium Bacillus anthracis. In this article, we report a highly sensitive detection techniqu e for pathogenic B. anthracis, based on the use of a surface-enhanced Raman scat- tering (SERS)-active magnetic sensor. Gold nanoparticles (GNPs) and magnetic beads were used as SERS nanotags and supporting substrates, respectively. Here, an SERS-based competitive immunoassay platform is described for the quantitative evaluation of the anthrax marker. Poly-γ- D-glutamic acid (PGA) capsules were used as a target biomarker for the highly sensitive detection of B. anthracis because PGA is known to be closely associated with the pathogenesis of B. anthracis infection. The SERS-based competitive assay reveals an extraordinarily high sensitivity for PGA with a limit of detection of ~1.0 ng/mL. This work provides a con- ceptually new immunoassay prototype for fast and sensitive detection of PGA markers.
URI
http://onlinelibrary.wiley.com/doi/10.1002/bkcs.10154/abstracthttp://hdl.handle.net/20.500.11754/21725
ISSN
1229-5949
DOI
http://dx.doi.org/10.1002/bkcs.10154
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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