Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 이수재 | - |
dc.date.accessioned | 2016-05-25T07:54:47Z | - |
dc.date.available | 2016-05-25T07:54:47Z | - |
dc.date.issued | 2015-01 | - |
dc.identifier.citation | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY, v. 167, Page. 15-23 | en_US |
dc.identifier.issn | 1532-0456 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/21351 | - |
dc.identifier.uri | http://www.sciencedirect.com/science/article/pii/S1532045614001082 | - |
dc.description.abstract | Ultraviolet B (UV-B) radiation presents an environmental hazard to aquatic organisms. To understand the molecular responses of the intertidal copepod Tigriopus japonicus to UV-B radiation, we measured the acute toxicity response to 96 h of UV-B radiation, and we also assessed the intracellular reactive oxygen species (ROS) levels, glutathione (GSH) content, and antioxidant enzyme (GST, GR, GPx, and SOD) activities after 24 h of exposure to UV-B with LD50 and half LD50 values. Also, expression patterns of p53 and hsp gene families with phosphorylation of p38 MAPK were investigated in UV-B-exposed copepods. We found that the ROS level, GSH content, and antioxidant enzyme activity levels were increased with the transcriptional upregulation of antioxidant-related genes, indicating that UV-B induces oxidative stress by generating ROS and stimulating antioxidant enzymatic activity as a defense mechanism. Additionally, we found that p53 expression was significantly increased after UV-B irradiation due to increases in the phosphorylation of the stress-responsive p38 MAPK, indicating that UV-B may be responsible for inducing DNA damage in T. japonicus. Of the hsp family genes, transcriptional levels of hsp20, hsp20.7, hsp70, and hsp90 were elevated in response to a low dose of UV-B radiation (9 kJ m(-2)), suggesting that these hsp genes may be involved in cellular protection against UV-B radiation. In this paper, we performed a pathway-oriented mechanistic analysis in response to UV-B radiation, and this analysis provides a better understanding of the effects of UV-B in the intertidal benthic copepod T. japonicus. (C) 2014 Elsevier Inc. All rights reserved. | en_US |
dc.description.sponsorship | We thank two anonymous reviewers for their constructive comments on the previous manuscript. This work was supported by a grant from the National Research Foundation (2012R1A2A2A02012617) to Jae-Seong Lee. | - |
dc.language.iso | en | en_US |
dc.publisher | ELSEVIER SCIENCE INC | en_US |
dc.subject | p53 | en_US |
dc.subject | Copepod; UV-B | en_US |
dc.subject | Tigriopus japonicus | en_US |
dc.subject | Oxidative stress | en_US |
dc.subject | p38 MAPK | en_US |
dc.subject | Heat shock protein | en_US |
dc.title | UV-B radiation-induced oxidative stress and p38 signaling pathway involvement in the benthic copepod Tigriopus japonicus | en_US |
dc.type | Article | en_US |
dc.relation.volume | 167 | - |
dc.identifier.doi | 10.1016/j.cbpc.2014.08.003 | - |
dc.relation.page | 15-23 | - |
dc.relation.journal | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY | - |
dc.contributor.googleauthor | Kim, Bo-Mi | - |
dc.contributor.googleauthor | Rhee, Jae-Sung | - |
dc.contributor.googleauthor | Lee, Kyun-Woo | - |
dc.contributor.googleauthor | Shin, Kyung-Hoon | - |
dc.contributor.googleauthor | Kim, Min-Jung | - |
dc.contributor.googleauthor | Lee, Young-Mi | - |
dc.contributor.googleauthor | Lee, Su-Jae | - |
dc.contributor.googleauthor | Lee, Jae-Seong | - |
dc.relation.code | 2015002029 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF NATURAL SCIENCES[S] | - |
dc.sector.department | DEPARTMENT OF LIFE SCIENCE | - |
dc.identifier.pid | sj0420 | - |
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