Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 류성언 | - |
dc.date.accessioned | 2016-05-24T06:59:11Z | - |
dc.date.available | 2016-05-24T06:59:11Z | - |
dc.date.issued | 2015-01 | - |
dc.identifier.citation | FEBS LETTERS, v. 589, NO 2, Page. 193-200 | en_US |
dc.identifier.issn | 0014-5793 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/21306 | - |
dc.identifier.uri | http://onlinelibrary.wiley.com/doi/10.1016/j.febslet.2014.11.046/abstract?systemMessage=Pay+Per+View+on+Wiley+Online+Library+will+be+unavailable+on+Saturday+15th+April+from+12%3A00-09%3A00+EDT+for+essential+maintenance.++Apologies+for+the+inconvenience. | - |
dc.description.abstract | To improve a previously constructed broadly neutralizing hepatitis B virus (HBV)-specific preS1 humanized antibody (HzKR127), we further humanized it through specificity-determining residue (SDR) grafting. Moreover, we improved affinity by mutating two residues in heavy-chain complementarity-determining regions (CDR), on the basis of the crystal structure of the antigen-antibody complex. HzKR127-3.2 exhibited 2.5-fold higher affinity and enhanced virus-neutralizing activity compared to the original KR127 antibody and showed less immunogenic potential than HzKR127. Enhanced virus-neutralizing activity was achieved by the increased association rate, providing insights into engineering potent antibody therapeutics for HBV immunoprophylaxis. HzKR127-3.2 may be a good candidate for HBV immunoprophylaxis. (C) 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. | en_US |
dc.language.iso | en | en_US |
dc.publisher | ELSEVIER SCIENCE BV | en_US |
dc.subject | Humanized antibody | en_US |
dc.subject | Specificity-determining residue grafting | en_US |
dc.subject | Affinity maturation | en_US |
dc.subject | Hepatitis B virus | en_US |
dc.subject | preS1 | en_US |
dc.subject | Virus neutralization | en_US |
dc.title | Enhanced humanization and affinity maturation of neutralizing anti-hepatitis B virus preS1 antibody based on antigen-antibody complex structure | en_US |
dc.type | Article | en_US |
dc.relation.no | 2 | - |
dc.relation.volume | 589 | - |
dc.identifier.doi | 10.1016/j.febslet.2014.11.046 | - |
dc.relation.page | 193-200 | - |
dc.relation.journal | FEBS LETTERS | - |
dc.contributor.googleauthor | Kim, Jin Hong | - |
dc.contributor.googleauthor | Gripon, Philippe | - |
dc.contributor.googleauthor | Bouezzedine, Fidaa | - |
dc.contributor.googleauthor | Jeong, Mun Sik | - |
dc.contributor.googleauthor | Chi, Seung-Wook | - |
dc.contributor.googleauthor | Ryu, Seong-Eon | - |
dc.contributor.googleauthor | Hong, Hyo Jeong | - |
dc.relation.code | 2015003652 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF ENGINEERING[S] | - |
dc.sector.department | DEPARTMENT OF BIOENGINEERING | - |
dc.identifier.pid | ryuse | - |
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