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dc.contributor.author김영필-
dc.date.accessioned2016-05-19T04:54:08Z-
dc.date.available2016-05-19T04:54:08Z-
dc.date.issued2015-01-
dc.identifier.citationANALYTICAL CHEMISTRY, v. 87, NO 2, Page. 1257-1265en_US
dc.identifier.issn0003-2700-
dc.identifier.urihttp://hdl.handle.net/20.500.11754/21225-
dc.identifier.urihttp://pubs.acs.org/doi/abs/10.1021/ac5039412-
dc.description.abstractWe report a rapid colorimetric assay to detect protein phosphatase (PP) activity based on the controlled assembly and disassembly of gold nanoparticles (AuNPs) via Zn(II)-specific coordination in the presence of His(6)-tagged phosphopeptides. Among divalent metal ions including Ni(II), Cu(II), Co(II), Mg(II), Mn(II), and Zn(II), only Zn(II) triggered a strong association between phosphopeptides with hexahistidine at a single end and nitrilotriacetic acid (NTA)-modified AuNPs (21.3 nm in core diameter), leading to the self-assembly of AuNPs and consequently changes in color of the AuNP solution. In contrast, unphosphorylated peptides and His6-deficient phosphopeptides did not change the color of the AuNP solution. As a result, protein phosphatase 1 (PP1) activity and its inhibition were easily quantified with high sensitivity by determining the extinction ratio (E-520/E-700) of colloidal AuNPs. Most importantly, this method was capable of detecting protein phosphatase 2A (PP2A) activity in immunoprecipitated plant extracts. Because PPs play pivotal roles in mediating diverse signal transduction pathways as primary effectors of protein dephosphorylation, we anticipate that our method will be applied as a rapid format method to analyze the activities of various PPs and their inhibition.en_US
dc.language.isoenen_US
dc.publisherAMER CHEMICAL SOCen_US
dc.titleRapid Detection of Protein Phosphatase Activity Using Zn(II)Coordinated Gold Nanosensors Based on His-Tagged Phosphopeptidesen_US
dc.typeArticleen_US
dc.relation.no2-
dc.relation.volume87-
dc.identifier.doi10.1021/ac5039412-
dc.relation.page1257-1265-
dc.relation.journalANALYTICAL CHEMISTRY-
dc.contributor.googleauthorLee, Jin Oh-
dc.contributor.googleauthorKim, Eun-Ji-
dc.contributor.googleauthorLim, Butaek-
dc.contributor.googleauthorKim, Tae-Wuk-
dc.contributor.googleauthorKim, Young-Pil-
dc.relation.code2015003190-
dc.sector.campusS-
dc.sector.daehakCOLLEGE OF NATURAL SCIENCES[S]-
dc.sector.departmentDEPARTMENT OF LIFE SCIENCE-
dc.identifier.pidypilkim-
Appears in Collections:
COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > LIFE SCIENCE(생명과학과) > Articles
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