1α,25-Dihydroxyvitamin D3 upregulates HIF-1 and TREM-1 via mTOR signaling

Title
1α,25-Dihydroxyvitamin D3 upregulates HIF-1 and TREM-1 via mTOR signaling
Authors
김태환
Keywords
TREM-1; 1α,25-dihydroxyviamin D3; mTOR; Monocytes; Macrophages
Issue Date
2015-01
Publisher
ELSEVIER SCIENCE BV
Citation
IMMUNOLOGY LETTERS, v. 163, NO 1, Page. 13-21
Abstract
•1,25(OH)2D3 upregulates hypoxia-inducible transcription factor (HIF) in human macrophages.•HIF is not mainly involved in 1,25(OH)2D3-induced TREM-1 expression.•1,25(OH)2D3 induces the expression of TREM-1 through the mTOR signaling pathway. Triggering receptor expressed on myeloid cells-1 (TREM-1) is induced by 1α,25-dihydroxyvitamin D3 (1,25(OH)2D3) in human monocytes/macrophages and epithelial cells. However, little information is available regarding the mechanism of 1,25(OH)2D3-induced TREM-1 expression in human monocytes/macrophages. In this study, 1,25(OH)2D3 was shown to strongly upregulate hypoxia-inducible transcription factor (HIF) in PMA-differentiated U937 cells. However, HIF was not mainly involved in 1,25(OH)2D3-induced TREM-1 expression. Instead, 1,25(OH)2D3-induced expression of TREM-1 was inhibited by rapamycin, a specific inhibitor of the mammalian target of rapamycin (mTOR) signaling pathway, indicating the involvement of mTOR. Induction of HIF proteins by 1,25(OH)2D3 was also inhibited by rapamycin. In addition, 1,25(OH)2D3 induced the phosphorylation of p70S6 kinase, a target of mTOR complex 1 (mTORC1). Our results suggest that 1,25(OH)2D3 induces the expression of TREM-1 through the mTOR signaling pathway in human macrophages.
URI
http://hdl.handle.net/20.500.11754/21050http://www.sciencedirect.com/science/article/pii/S0165247814002582
ISSN
0165-2478
DOI
http://dx.doi.org/10.1016/j.imlet.2014.11.004
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COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
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