Isolation of Alkaloids from Sinomenium acutum by Centrifugal Partition Chromatography and Their Ameliorating Effects on Dexamethasone-Induced Atrophy in C2C12 Myotubes
- Title
- Isolation of Alkaloids from Sinomenium acutum by Centrifugal Partition Chromatography and Their Ameliorating Effects on Dexamethasone-Induced Atrophy in C2C12 Myotubes
- Author
- 김철영
- Keywords
- acutumine; centrifugal partition chromatography; magnoflorine; sinomenine; Sinomenium acutum; skeletal muscle atrophy
- Issue Date
- 2023-09
- Publisher
- Multidisciplinary Digital Publishing Institute (MDPI)
- Citation
- Separations, v. 10, NO. 9, article no. 470, Page. 1.0-12.0
- Abstract
- Bioactivity-guided isolation was conducted using centrifugal partition chromatography (CPC) from an extract of Sinomenium acutum rhizome, which has shown promising preventive effects in a dexamethasone-induced C2C12 myotube atrophy model. CPC was operated with a solvent system of n-butanol–acetonitrile–water (10:2:8, v/v/v, containing 0.5% triethylamine) in dual mode (ascending to descending), which provided a high recovery rate (>99%) with a high resolution. Then, the preventive effects of the obtained CPC fractions were examined against dexamethasone-induced atrophy in C2C12 myotubes according to the weight ratios of the obtained fractions. The active fractions were further purified by semi-preparative HPLC that led to obtaining five alkaloids, one lignan glycoside, and one phenylpropanoid glycoside. Among these, at a concentration of 1 nM, sinomenine, magnoflorine, and acutumine could ameliorate dexamethasone-induced myotube atrophy in C2C12 myotubes by 9.3%, 13.8%, and 11.3%, respectively. © 2023 by the authors.
- URI
- https://www.scopus.com/record/display.uri?eid=2-s2.0-85172300745&origin=inward&txGid=61a034bb2fcb48f93c64059e25cb0d32https://repository.hanyang.ac.kr/handle/20.500.11754/187783
- ISSN
- 2297-8739;2297-8739
- DOI
- 10.3390/separations10090470
- Appears in Collections:
- COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
- Files in This Item:
- 109249_김철영.pdfDownload
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