Poly(dimethyl siloxane)-based protein chip for simultaneous detection of multiple samples: Use of glycidyl methacrylate photopolymer for site-specific protein immobilization
- Title
- Poly(dimethyl siloxane)-based protein chip for simultaneous detection of multiple samples: Use of glycidyl methacrylate photopolymer for site-specific protein immobilization
- Author
- 성기훈
- Keywords
- glycidyl methacrylate; poly(dimethyl siloxane) (PDMS); microfluidics; poly(ethylene glycol) diacrylate; biomolecular immobilization
- Issue Date
- 2006-12
- Publisher
- ELSEVIER ADVANCED TECHNOLOGY
- Citation
- BIOSENSORS & BIOELECTRONICS, v. 22, NO. 5, Page. 613-620
- Abstract
- This paper describes fabrication of a poly(dimethyl siloxane) (PDMS)-based chip to analyze multiple protein interactions utilizing glycidyl methacrylate (GMA) photopolymer for a site-specific immobilization of capture proteins in a closed system. First, using one direction channels of a PDMS mold having cross-channels, GMA micropads were prepared by photopolymerizing GMA solution by 365 nm light irradiation at predetermined positions. After the first mold was replaced with a second mold having higher height or directly without mold changing, capture proteins were allowed to be covalently immobilized onto the surface of the epoxide-activated GMA pads. Following immobilization, poly(ethylene glycol) diacrylate (PEG-DA) precursor was photopolymerized at specific regions to generate plugs for prevention of mixing between different sample injection channels, diminishing the need of a mold changing for sample injections. Final chip was assembled by connecting separated sample injection channels using a connector mold. The viability of this strategy was successfully demonstrated by simultaneous detection of two different antigen-antibody interactions. (c) 2006 Elsevier B.V. All rights reserved.
- URI
- https://www.sciencedirect.com/science/article/pii/S0956566306000509?via%3Dihubhttps://repository.hanyang.ac.kr/handle/20.500.11754/183590
- ISSN
- 0956-5663;1873-4235
- DOI
- 10.1016/j.bios.2006.01.029
- Appears in Collections:
- COLLEGE OF ENGINEERING SCIENCES[E](공학대학) > BIONANO ENGINEERING(생명나노공학과) > Articles
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