Patulin Removal Mechanism of Meyerozyma guilliermondii ATCC 6260 A-Y3 isolated from apples

Abstract

The contamination of patulin in food has become an important factor threatening the health of consumers and bringing economic losses to food producers. Therefore, the prevention and control of patulin contamination in food cannot be ignored. Biocontrol of patulin has the advantages of economy, high efficiency and safety. It has become a hot topic in academic research and shows broad application prospects. As a common biological control agent, yeast has a potential application in the prevention and treatment of patulin. In this study, a yeast strain with efficient removal characteristics of patulin was screened and identified, and its removal characteristics and removal mechanism were studied. The results of this study are as follows: 1. There were 4 yeasts screened from apples, one of which could efficiently remove patulin, and the yeast could achieve a removal rate of 91.69% at 48 h (the concentration of patulin was 1μg/mL). The yeast strain was identified as Meyerozyma guilliermondii ATCC 6260, named A-Y3. 2. The removal characteristics of patulin by M.guilliermondii A-Y3 were studied. M. guilliermondii A-Y3 had a high removal rate within 48 h and reached 100% at 72 h. Through the removal rate, it was found that patulin was rapidly removed in the first 6 h, and the removal rate increased after 6 h but the rate slowed down. At 35℃, the fermentation broth added with M.guilliermondii A-Y3 had the highest removal rate of patulin after 48 h of culture, which was the best temperature. With the increase of initial concentration of patulin, the ability of M.guilliermondii A-Y3 to remove patulin decreased gradually. 3. The mechanism of M.guilliermondii A-Y3 removing patulin was preliminarily studied. Research findings, both yeast cells and cell walls of M.guilliermondii A-Y3 could not adsorb patulin. So extracellular metabolites of M.guilliermondii A-Y3 does not degrade patulin, while intracellular enzymes of M.guilliermondii A-Y3 have the ability to degrade patulin.