In vitro culture of hematopoietic stem cell niche using angiopoietin-1-coupled alginate hydrogel
- Title
- In vitro culture of hematopoietic stem cell niche using angiopoietin-1-coupled alginate hydrogel
- Author
- 이상경
- Keywords
- Hematopoietic stem cell; Synthetic niche; Angiopoietin-1; Alginate hydrogel
- Issue Date
- 2022-06
- Publisher
- Elsevier B.V.
- Citation
- International Journal of Biological Macromolecules, v. 209, Page. 1893-1899
- Abstract
- Stem cells exist and maintain their quiescence and pluripotency in stem cell niche. Here, we hypothesized that regulation of cell-cell interactions using a polymeric scaffold as synthetic extracellular matrix (ECM) could be critical in creating a hematopoietic stem cell (HSC) niche in vitro. Angiopoietin-1 (Ang1) binds to the tyrosine kinase receptor (Tie2), and regulation of the Tie2/Ang1 interaction is important in maintaining the quiescence of HSCs in vivo. Alginate hydrogel was thus modified with Ang1 as a synthetic ECM to mimic the HSC niche. Long-term HSCs (CD34−, CD135−, and CD150+) were isolated from mouse femurs and cultured on Ang1-modified alginate hydrogel. The percentage of LT-HSCs in G0 phase was 46.8 ± 1.8%, which was comparable to that of LT-HSCs co-cultured with osteoblasts (46.8 ± 2.1%). Ang1-coupled alginate gels were useful to provide a niche for HSC quiescence without a co-culture system. Polymeric scaffolds containing biomimetic and cell-instructive characteristics for stem cell phenotype regulation might help create HSC niches in vitro and be useful to engineer tissues and transplant stem cells.
- URI
- https://www.sciencedirect.com/science/article/pii/S0141813022008777?via%3Dihubhttps://repository.hanyang.ac.kr/handle/20.500.11754/178155
- ISSN
- 0141-8130;1879-0003
- DOI
- 10.1016/j.ijbiomac.2022.04.163
- Appears in Collections:
- COLLEGE OF ENGINEERING[S](공과대학) > BIOENGINEERING(생명공학과) > Articles
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