Plasmid DNA Nanoparticles for Nonviral Oral Gene Therapy
- Title
- Plasmid DNA Nanoparticles for Nonviral Oral Gene Therapy
- Author
- 이동윤
- Keywords
- diabetes; gene therapy; glucagon-like peptide 1; oral delivery; bile acids; protein replacement therapy
- Issue Date
- 2021-06
- Publisher
- American Chemical Society
- Citation
- Nano Letters, v. 21, NO. 11, Page. 4666-4675
- Abstract
- Herein, a bile acid-inspired triple padlock oral gene delivery platform is developed, facilitating the protection of the therapeutic gene from gastrointestinal degradation, selective intestinal accumulation through a bile acid-specific transporter, and transportation of pDNA NPs through the enterohepatic recycling system. This nonviral oral gene delivery nanoparticle exhibits excellent gene expression kinetics in in vitro, in vivo, and ex vivo studies. A single oral dose leads to maintaining normoglycemia for up to 7 days in three different diabetes mouse models and 14 days in diabetic monkeys. Also, the optimized dosage form can reduce nonfast blood glucose levels and hemoglobin A1C within a normal range from the last stage diabetes conditions with a reduction of weight gain from changes of food uptake behavior after treatment once weekly for 20 weeks. Taken together, the current findings could improve the current painful treatment experience of diabetics and thus improve their quality of life. © 2021 American Chemical Society. All rights reserved.
- URI
- https://pubs.acs.org/doi/10.1021/acs.nanolett.1c00832https://repository.hanyang.ac.kr/handle/20.500.11754/178144
- ISSN
- 1530-6984;1530-6992
- DOI
- 10.1021/acs.nanolett.1c00832
- Appears in Collections:
- COLLEGE OF ENGINEERING[S](공과대학) > BIOENGINEERING(생명공학과) > Articles
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