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장목만에서 분리한 유독 와편모조류 Alexandrium pacificum (Group IV): 형태, 계통 분류와 온도, 염분 및 영양염 농도 차이에 따른 성장 변화

Title
장목만에서 분리한 유독 와편모조류 Alexandrium pacificum (Group IV): 형태, 계통 분류와 온도, 염분 및 영양염 농도 차이에 따른 성장 변화
Other Titles
Alexandrium pacificum (Group IV) isolated from Jangmok Bay, Korea: Morphology, phylogeny, and effects of temperature, salinity, and nutrient levels on growth
Author
이원철
Keywords
Alexandrium; morphology; phylogeny; nutrient; growth
Issue Date
2022-09
Publisher
한국환경생물학회
Citation
환경생물, v. 40, NO. 3, Page. 290-300
Abstract
장목만에서 분리한 Alexandrium 종의 배양주를 확보하여 형태와 계통분류를 통해 종을 명확히 하고 온도, 염분 및 영양염 농도 차이에 따른 성장 반응을 파악하였다. 확보한 Alexandrium 종은 형태적으로 Alexandrium catenella, A. tamarense와 구분이 되지 않았다. 하지만, 분자계통도 작성을 통해 장목만에서 분리한 Alexandrium 종은 A. tamarense species complex 내의 A. pacificum (Group IV)에 속하는 것을 확인할 수 있었다. 온도에 대한 성장 반응 실험에서 A. pacificum (Group IV)는 15°C와 20°C에서 높은 성장 속도와 유영세포 농도를 보였고, 염분에 대한 성장 반응 실험에서 A. pacificum (Group IV)은 염분 20~35 psu의 넓은 염분 범위에서 성장하였다. 즉, A. pacificum (Group IV)은 협온성, 광염성의 특징을 가진다. 그리고 영양염 첨가에 따른 성장 반응 실험에서, A. pacificum (Group IV)은 질산염과 인산염의 농도 증가와 함께 성장을 하였지만, 질산염과 인산염에 대해 다른 소비 전략을 가지는 것으로 나타났다.;A strain of Alexandrium species was established by isolating cells from Jangmok Bay, Korea. Its morphology and molecular phylogeny based on LSU rRNA gene sequences were examined. In addition, growth responses of this Alexandrium species to changes in temperature, salinity, and nutrient concentrations were investigated. This Alexandrium species from Jangmok Bay had a ventral pore on the 1′, which was morphologically consistent with previously described Alexandrium tamarense and A. catenella. Phylogenetic analyses revealed that this isolate was assigned to A. pacificum (Group IV) within A. tamarense species complex. In growth experiments, relatively high growth rates and cell densities of A. pacificum (Group IV) were observed at 15°C and 20°C. This species also grew under a wide range of salinity. This indicates that this Korean isolate of A. pacificum (Group IV) is a stenothermic and euryhaline species. In growth responses to changes in nutrient levels, enhanced growth rates and cell densities of A. pacificum (Group IV) were observed with additions of nitrate and phosphate. In particular, rapid uptakes of phosphate by A. pacificum (Group IV) were observed in experimental treatments, indicating that the increase in phosphate concentration could stimulate the growth of A. pacificum (Group IV).
URI
http://www.ebr.or.kr/journal/article.php?code=84488https://repository.hanyang.ac.kr/handle/20.500.11754/177901
ISSN
1226-9999;2287-7851
DOI
10.11626/KJEB.2022.40.3.290
Appears in Collections:
COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > LIFE SCIENCE(생명과학과) > Articles
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