Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김영필 | - |
dc.date.accessioned | 2022-12-05T00:14:14Z | - |
dc.date.available | 2022-12-05T00:14:14Z | - |
dc.date.issued | 2022-02 | - |
dc.identifier.citation | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 23, NO. 3, article no. 1605, Page. 1-12 | en_US |
dc.identifier.issn | 1661-6596;1422-0067 | en_US |
dc.identifier.uri | https://www.mdpi.com/1422-0067/23/3/1605 | en_US |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/177875 | - |
dc.description.abstract | Activity-based monitoring of cell-secreted proteases has gained significant interest due to the implication of these substances in diverse cellular functions. Here, we demonstrated a cell-based method of monitoring protease activity using fluorescent cell-permeable peptides. The activatable peptide consists of anionic (EEEE), cleavable, and cationic sequences (RRRR) that enable intracellular delivery by matrix metalloproteinase-2 (MMP2), which is secreted by living cancer cells. Compared to HT-29 cells (MMP2-negative), HT-1080 cells (MMP2-positive) showed a strong fluorescence response to the short fluorescent peptide via cell-secreted protease activation. Our approach is expected to find applications for the rapid visualization of protease activity in living cells. | en_US |
dc.description.sponsorship | This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIT) (No. 2019R1A2C2004858); the Basic Science Research Programs (2020R1A6A1A06046728) through the NRF, funded by the Ministry of Education; the KIST Institutional Program (Project No. 2Z06270-20-137); and the Korea Environment Industry and Technology Institute (KEITI) through the Aquatic Ecosystem Conservation Research Program, funded by the Korea Ministry of Environment (MOE) (No. 2020003030007). | en_US |
dc.language | en | en_US |
dc.publisher | MDPI | en_US |
dc.source | 84049_김영필.pdf | - |
dc.subject | activatable peptides | en_US |
dc.subject | fluorescent imaging | en_US |
dc.subject | proteases | en_US |
dc.subject | MMP2 | en_US |
dc.subject | cancer cells | en_US |
dc.title | Activatable Peptides for Rapid and Simple Visualization of Protease Activity Secreted in Living Cells | en_US |
dc.type | Article | en_US |
dc.relation.no | 3 | - |
dc.relation.volume | 23 | - |
dc.identifier.doi | 10.3390/ijms23031605 | en_US |
dc.relation.page | 1-12 | - |
dc.relation.journal | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.contributor.googleauthor | Kim, Gae-Baik | - |
dc.contributor.googleauthor | Lee, Jeong Min | - |
dc.contributor.googleauthor | Nguyen, Duc Long | - |
dc.contributor.googleauthor | Lee, Joonseok | - |
dc.contributor.googleauthor | Kim, Young-Pil | - |
dc.sector.campus | S | - |
dc.sector.daehak | 자연과학대학 | - |
dc.sector.department | 생명과학과 | - |
dc.identifier.pid | ypilkim | - |
dc.identifier.orcid | https://orcid.org/0000-0001-7234-1320 | - |
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