Selective optosensing of iron(III) ions in HeLa cells using NaYF4:Yb3+/Tm3+ upconversion nanoparticles coated with polyepinephrine
- Title
- Selective optosensing of iron(III) ions in HeLa cells using NaYF4:Yb3+/Tm3+ upconversion nanoparticles coated with polyepinephrine
- Author
- GERELKHUUZAYAKHUU
- Keywords
- HeLa cell; Epinephrine; Ferric ions; Upconversion; Intracellular imaging
- Issue Date
- 2021-01
- Publisher
- SPRINGER HEIDELBERG
- Citation
- ANALYTICAL AND BIOANALYTICAL CHEMISTRY, v. 413, no. 5, page. 1363-1371
- Abstract
- Novel polyepinephrine-modified NaYF4:Yb,Tm upconversion luminescent nanoparticles (UCNP@PEP) were prepared via the self-polymerization of epinephrine on the surfaces of the UCNPs for selective sensing of Fe3+ inside a cell and for intracellular imaging. The proposed UCNP@PEP probe is a strong blue light emitter (λmax = 474 nm) upon exposure to an excitation wavelength of 980 nm. The probe was used for detecting Fe3+ owing to the complexation reaction between UCNP@PEP and Fe3+, resulting in reduced upconversion luminescence (UCL) intensity. The proposed probe has a detection limit of 0.2 μM and a good linear range of 1–10 μM for sensing Fe3+ ions. Moreover, the UCNP@PEP probe displays high cell viability (90%) and is feasible for intracellular imaging. The ability of the probe to sense Fe3+ in a human serum sample was tested and shows promising output for diagnostic purposes. The prepared UCNP@PEP probe was characterized by using UV-visible (UV-Vis) absorption spectrometry, fluorescence (FL) spectrometry, field emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS), and Fourier transform infrared spectroscopy (FT-IR).
- URI
- https://link.springer.com/article/10.1007/s00216-020-03099-1https://repository.hanyang.ac.kr/handle/20.500.11754/175509
- ISSN
- 1618-2642 ; 1618-2650
- DOI
- 10.1007/s00216-020-03099-1
- Appears in Collections:
- OFFICE OF ACADEMIC AFFAIRS[S](교무처) > Center for Creative Convergence Education(창의융합교육원) > Articles
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