Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 성기훈 | - |
dc.date.accessioned | 2022-07-27T00:57:01Z | - |
dc.date.available | 2022-07-27T00:57:01Z | - |
dc.date.issued | 2021-04 | - |
dc.identifier.citation | MICROCHIMICA ACTA, v. 188, NO 4, Page. 1-10 | en_US |
dc.identifier.issn | 0026-3672 | - |
dc.identifier.uri | https://link.springer.com/article/10.1007/s00604-021-04775-4 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/171764 | - |
dc.description.abstract | The in situ synthesis is reported of citric acid-functionalized ultra-fine bimetallic PtRu alloy nanoparticles (CA@PtRu ANPs) through a simple one-pot wet chemical method. The cost-efficient CA@PtRu ANPs with an average diameter of 3.2 nm revealed to have enhanced surface area, peroxidase-like activity, high stability, and adequate availability of functional groups to bind biomolecules. Along with nanoparticle surface area, the surface charge has also significantly affected the peroxidase-like activity and the colloidal suspension stability. As an excellent immobilization matrix and peroxidase mimic, the CA@PtRu ANPs were utilized to develop non-enzymatic colorimetric immunoassay for rapid, selective, and sensitive quantification of C-reactive protein (CRP) biomarkers. In this immunoassay, CA@PtRu ANPs serve as enzyme mimic that significantly amplifies the color signals, and amine-functionalized silica-coated magnetic microbeads (APTES/SiO2@Fe3O4) act as CRP-recognizing capture probes. The absorbance curves of colorimetric immunoassay were measured in wavelengths between 550 and 750 nm, and the maximum absorbance at 652 nm was used to establish a linear relationship between absorbance and CRP concentrations. The developed colorimetric immunoassay showed rapid and sensitive quantification of CRP levels from 0.01 to 180 mu g mL(-1) with a LOD of 0.01 mu g mL(-1). Moreover, the mean recovery of CRP from spiked human serum samples lies between 97 and 109% (n = 3), which indicates that the proposed nanozyme-linked immunoassay has the potential to be used in rapid point-of-care applications. | en_US |
dc.description.sponsorship | The authors acknowledge the Ministry of Education for their funding support through Basic Science Research Program under National Research Foundation of Korea (NRF-2018R1A6A1A03024231). | en_US |
dc.language.iso | en | en_US |
dc.publisher | SPRINGER WIEN | en_US |
dc.subject | Bimetallic alloy | en_US |
dc.subject | Nanozyme | en_US |
dc.subject | Immunoassay | en_US |
dc.subject | C-reactive protein | en_US |
dc.subject | Colorimetric detection | en_US |
dc.title | Functionalized ultra-fine bimetallic PtRu alloy nanoparticle with high peroxidase-mimicking activity for rapid and sensitive colorimetric quantification of C-reactive protein | en_US |
dc.type | Article | en_US |
dc.relation.no | 4 | - |
dc.relation.volume | 188 | - |
dc.identifier.doi | 10.1007/s00604-021-04775-4 | - |
dc.relation.page | 1-10 | - |
dc.relation.journal | MICROCHIMICA ACTA | - |
dc.contributor.googleauthor | Gupta, Pramod K. | - |
dc.contributor.googleauthor | Son, Seong Eun | - |
dc.contributor.googleauthor | Seong, Gi Hun | - |
dc.relation.code | 2021008838 | - |
dc.sector.campus | E | - |
dc.sector.daehak | COLLEGE OF ENGINEERING SCIENCES[E] | - |
dc.sector.department | DEPARTMENT OF BIONANO ENGINEERING | - |
dc.identifier.pid | ghseong | - |
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