Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 진언선 | - |
dc.date.accessioned | 2022-04-15T01:34:35Z | - |
dc.date.available | 2022-04-15T01:34:35Z | - |
dc.date.issued | 2020-08 | - |
dc.identifier.citation | SCIENTIFIC REPORTS, v. 10, no. 1, article no. 13947 | en_US |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | https://www.nature.com/articles/s41598-020-70503-2 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/170015 | - |
dc.description.abstract | Microalgae not only serve as raw materials for biofuel but also have uses in the food, pharmaceutical, and cosmetic industries. However, regulated gene expression in microalgae has only been achieved in a few strains due to the lack of genome information and unstable transformation. This study developed a species-specific transformation system for an oleaginous microalga, Ettlia sp. YC001, using electroporation. The electroporation was optimized using three parameters (waveform, field strength, and number of pulses), and the final selection was a 5 kV cm(-1) field strength using an exponential decay wave with one pulse. A new strong endogenous promoter CRT (Pcrt) was identified using transcriptome and quantitative PCR analysis of highly expressed genes during the late exponential growth phase. The activities of this promoter were characterized using a codon optimized cyan fluorescent protein (CFP) as a reporter. The expression of CFP was similar under Pcrt and under the constitutive promoter psaD (PpsaD). The developed transformation system using electroporation with the endogenous promoter is simple to prepare, is easy to operate with high repetition, and utilizes a species-specific vector for high expression. This system could be used not only in molecular studies on microalgae but also in various industrial applications of microalgae. | en_US |
dc.description.sponsorship | This research was supported equally by the Advanced Biomass R&D Center, a Global Frontier Program, funded by the Korean Ministry of Science, ICT and Future Planning (2010-0029723) and the National Research Foundation of Korea (NRF) in grant funded by the Korean government (NRF-2018R1C1B3009513). | en_US |
dc.language.iso | en | en_US |
dc.publisher | NATURE PUBLISHING GROUP | en_US |
dc.subject | ALGA CHLAMYDOMONAS-REINHARDTII | en_US |
dc.subject | EXPRESSION | en_US |
dc.subject | CHLOROPHYTA | en_US |
dc.subject | GENE | en_US |
dc.title | Development of a species-specific transformation system using the novel endogenous promoter calreticulin from oleaginous microalgae Ettlia sp. | en_US |
dc.type | Article | en_US |
dc.relation.no | 1 | - |
dc.relation.volume | 10 | - |
dc.identifier.doi | 10.1038/s41598-020-70503-2 | - |
dc.relation.page | 1-12 | - |
dc.relation.journal | SCIENTIFIC REPORTS | - |
dc.contributor.googleauthor | Lee, Jun-Woo | - |
dc.contributor.googleauthor | Lee, Min-Woo | - |
dc.contributor.googleauthor | Ha, Ji-San | - |
dc.contributor.googleauthor | Kim, Dae-Soo | - |
dc.contributor.googleauthor | Jin, EonSeon | - |
dc.contributor.googleauthor | Lee, Hyung-Gwan | - |
dc.contributor.googleauthor | Oh, Hee-Mock | - |
dc.relation.code | 2020051242 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF NATURAL SCIENCES[S] | - |
dc.sector.department | DEPARTMENT OF LIFE SCIENCE | - |
dc.identifier.pid | esjin | - |
dc.identifier.orcid | https://orcid.org/0000-0001-5691-0124 | - |
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