Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 장수화 | - |
dc.date.accessioned | 2022-03-24T07:14:19Z | - |
dc.date.available | 2022-03-24T07:14:19Z | - |
dc.date.issued | 2020-07 | - |
dc.identifier.citation | FRONTIERS IN IMMUNOLOGY, v. 11, article no. 1436 | en_US |
dc.identifier.issn | 1664-3224 | - |
dc.identifier.uri | https://www.frontiersin.org/articles/10.3389/fimmu.2020.01436/full | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/169393 | - |
dc.description.abstract | Proper expression of the transcription factor, Positive regulatory domain 1 (PRDM1), is required for maintaining homeostasis of human monocyte derived-dendritic cells (MO-DCs). The molecular mechanisms and gene targets of PRDM1 in B and T lymphocytes have been identified. However, the function of PRDM1 in dendritic cells (DCs) remains unclear. We investigate co-regulators of PRDM1 in MO-DCs identified by mass spectrometry (MS) and co-immunoprecipitation (Co-IP). Notably, non-POU domain-containing octamer-binding protein (NonO) was found to be a PRDM1 binding protein in the nucleus of MO-DCs. NonO is recruited to the PRDM1 binding site in the promoter region of IL-6. Knockdown of NonO expression by siRNA lessened suppression of IL-6 promoter activity by PRMD1 following LPS stimulation. While NonO binding to PRDM1 was observed in human myeloma cell lines, an effect of NonO on IL-6 expression was not observed. Thus, loss of NonO interrupted the inhibitory effect of PRDM1 on IL-6 expression in MO-DCs, but not plasma cells. Moreover, MO-DCs with low expression of PRDM1 or NonO induce an increased number of IL-21-producing T-FH-like cellsin vitro. These data suggest that low level of PRDM1 and NonO lead to enhanced activation of MO-DCs and the regulation of MO-DC function by PRDM1 is mediated through cell lineage-specific mechanisms. | en_US |
dc.description.sponsorship | This work was supported by a grant from the US National Institute of Health (R01 AR065209 to SK, SJ, HT, and KL). | en_US |
dc.language.iso | en | en_US |
dc.publisher | FRONTIERS MEDIA SA | en_US |
dc.subject | PRDM1 | en_US |
dc.subject | NonO | en_US |
dc.subject | IL-6 | en_US |
dc.subject | inflammation | en_US |
dc.subject | dendritic cells | en_US |
dc.title | NonO Is a Novel Co-factor of PRDM1 and Regulates Inflammatory Response in Monocyte Derived-Dendritic Cells | en_US |
dc.type | Article | en_US |
dc.relation.volume | 11 | - |
dc.identifier.doi | 10.3389/fimmu.2020.01436 | - |
dc.relation.page | 1-13 | - |
dc.relation.journal | FRONTIERS IN IMMUNOLOGY | - |
dc.contributor.googleauthor | Lee, Kyungwoo | - |
dc.contributor.googleauthor | Jang, Su Hwa | - |
dc.contributor.googleauthor | Tian, Hong | - |
dc.contributor.googleauthor | Kim, Sun Jung | - |
dc.relation.code | 2020050760 | - |
dc.sector.campus | S | - |
dc.sector.daehak | RESEARCH INSTITUTE[S] | - |
dc.sector.department | HBRI | - |
dc.identifier.pid | jsh8209 | - |
dc.identifier.orcid | https://orcid.org/0000-0002-3022-6612 | - |
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