Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김정목 | - |
dc.date.accessioned | 2021-11-30T01:41:13Z | - |
dc.date.available | 2021-11-30T01:41:13Z | - |
dc.date.issued | 2020-05 | - |
dc.identifier.citation | ANALYTICAL CHEMISTRY, v. 92, no. 9, page. 6462-6469 | en_US |
dc.identifier.issn | 0003-2700 | - |
dc.identifier.issn | 1520-6882 | - |
dc.identifier.uri | https://pubs.acs.org/doi/10.1021/acs.analchem.9b05653 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/166521 | - |
dc.description.abstract | The field of terminal proteomics is limited in that it is optimized for large-scale analysis via multistep processes involving liquid chromatography. Here, we present an integrated N-terminal peptide enrichment method (iNrich) that can handle as little as 25 mu g of cell lysate via a single-stage encapsulated solid-phase extraction column. iNrich enables simple, rapid, and reproducible sample processing, treatment of a wide range of protein amounts (25 mu g similar to 1 mg), multiplexed parallel sample preparation, and in-stage sample prefractionation using a mixed-anion-exchange filter. We identified similar to 5000 N-terminal peptides (Nt-peptides) from only 100 mu g of human cell lysate including Nt-formyl peptides. Multiplexed sample preparation facilitated quantitative and robust enrichment of N-terminome iNrich with dozens of samples simultaneously. We further developed the method to incorporate isobaric tags such as a tandem mass tag (TMT) and used it to discover novel peptides during ER stress analysis. The iNrich facilitated high-throughput N-terminomics and degradomics at a low cost using commercially available reagents and apparatus, without requiring arduous procedures. | en_US |
dc.description.sponsorship | The study was supported by grants from NRF (2020R1A2C2003685, 2017M3A9F9030559) and KHIDI (HI14C3484) and an institutional grant from KIST. | en_US |
dc.language.iso | en | en_US |
dc.publisher | AMER CHEMICAL SOC | en_US |
dc.subject | PROTEOLYTIC EVENTS | en_US |
dc.subject | PEPTIDES | en_US |
dc.subject | PROTEINS | en_US |
dc.subject | TERMINOMICS | en_US |
dc.subject | INHIBITION | en_US |
dc.subject | ACTIVATION | en_US |
dc.subject | APOPTOSIS | en_US |
dc.subject | PROJECT | en_US |
dc.subject | TAILS | en_US |
dc.title | iNrich, Rapid and Robust Method to Enrich N-Terminal Proteome in a Highly Multiplexed Platform | en_US |
dc.type | Article | en_US |
dc.relation.no | 9 | - |
dc.relation.volume | 92 | - |
dc.identifier.doi | 10.1021/acs.analchem.9b05653 | - |
dc.relation.page | 6462-6469 | - |
dc.relation.journal | ANALYTICAL CHEMISTRY | - |
dc.contributor.googleauthor | Ju, Shinyeong | - |
dc.contributor.googleauthor | Kwon, Yumi | - |
dc.contributor.googleauthor | Kim, Jeong-Mok | - |
dc.contributor.googleauthor | Park, Daechan | - |
dc.contributor.googleauthor | Lee, Seonjeong | - |
dc.contributor.googleauthor | Lee, Jin-Won | - |
dc.contributor.googleauthor | Hwang, Cheol-Sang | - |
dc.contributor.googleauthor | Lee, Cheolju | - |
dc.relation.code | 2020048062 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF NATURAL SCIENCES[S] | - |
dc.sector.department | DEPARTMENT OF LIFE SCIENCE | - |
dc.identifier.pid | jmokkim | - |
dc.identifier.orcid | https://orcid.org/0000-0002-7223-248X | - |
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