Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 심지원 | - |
dc.date.accessioned | 2021-11-23T07:57:24Z | - |
dc.date.available | 2021-11-23T07:57:24Z | - |
dc.date.issued | 2020-05 | - |
dc.identifier.citation | ELIFE, v. 9, article no. e54818 | en_US |
dc.identifier.issn | 2050-084X | - |
dc.identifier.uri | https://elifesciences.org/articles/54818 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/166414 | - |
dc.description.abstract | Drosophila blood cells, called hemocytes, are classified into plasmatocytes, crystal cells, and lamellocytes based on the expression of a few marker genes and cell morphologies, which are inadequate to classify the complete hemocyte repertoire. Here, we used single-cell RNA sequencing (scRNA-seq) to map hemocytes across different inflammatory conditions in larvae. We resolved plasmatocytes into different states based on the expression of genes involved in cell cycle, antimicrobial response, and metabolism together with the identification of intermediate states. Further, we discovered rare subsets within crystal cells and lamellocytes that express fibroblast growth factor (FGF) ligand branchless and receptor breathless, respectively. We demonstrate that these FGF components are required for mediating effective immune responses against parasitoid wasp eggs, highlighting a novel role for FGF signaling in inter-hemocyte crosstalk. Our scRNA-seq analysis reveals the diversity of hemocytes and provides a rich resource of gene expression profiles for a systems-level understanding of their functions. | en_US |
dc.description.sponsorship | Samsung Science and Technology Foundation SSTF-BA1701-15 Jiwon Shim Howard Hughes Medical Institute Norbert Perrimon Harvard Medical School Victor Barrera Michael J Steinbaugh Shannan Ho Sui The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication. | en_US |
dc.language.iso | en | en_US |
dc.publisher | ELIFE SCIENCES PUBLICATIONS LTD | en_US |
dc.subject | IMMUNE-RESPONSE | en_US |
dc.subject | HEMATOPOIETIC STEM | en_US |
dc.subject | INNATE IMMUNITY | en_US |
dc.subject | RIBOSOMAL-RNA | en_US |
dc.subject | CYCLE EXIT | en_US |
dc.subject | DIFFERENTIATION | en_US |
dc.subject | GENE | en_US |
dc.subject | EXPRESSION | en_US |
dc.subject | HEMOCYTES | en_US |
dc.subject | REVEALS | en_US |
dc.title | A single-cell survey of Drosophila blood | en_US |
dc.type | Article | en_US |
dc.relation.volume | 9 | - |
dc.identifier.doi | 10.7554/eLife.54818 | - |
dc.relation.page | 1-15 | - |
dc.relation.journal | ELIFE | - |
dc.contributor.googleauthor | Tattikota, Sudhir Gopal | - |
dc.contributor.googleauthor | Cho, Bumsik | - |
dc.contributor.googleauthor | Liu, Yifang | - |
dc.contributor.googleauthor | Hu, Yanhui | - |
dc.contributor.googleauthor | Barrera, Victor | - |
dc.contributor.googleauthor | Steinbaugh, Michael J. | - |
dc.contributor.googleauthor | Yoon, Sang-Ho | - |
dc.contributor.googleauthor | Comjean, Aram | - |
dc.contributor.googleauthor | Li, Fangge | - |
dc.contributor.googleauthor | Shim, Jiwon | - |
dc.relation.code | 2020046099 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF NATURAL SCIENCES[S] | - |
dc.sector.department | DEPARTMENT OF LIFE SCIENCE | - |
dc.identifier.pid | jshim | - |
dc.identifier.researcherID | AAQ-4501-2021 | - |
dc.identifier.orcid | https://orcid.org/0000-0003-2409-1130 | - |
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