The downregulation of Bcl-2 expression is necessary for theophylline-induced apoptosis of eosinophils.
- Title
- The downregulation of Bcl-2 expression is necessary for theophylline-induced apoptosis of eosinophils.
- Author
- 정일엽
- Issue Date
- 2000-08
- Publisher
- Academic Press
- Citation
- Cellular Immunology, v. 203, Issue. 2, page. 95-102
- Abstract
- Apoptosis of eosinophils is of increasingly important value in modulating allergic inflammatory airway diseases, such as asthma, and is suppressed by interleukin-5 (IL-5) in in vitro culture. In this study, we examined the effects of theophylline on survival/apoptosis, intracellular cAMP concentration, and Bcl-2 protein expression. Treatment with theophylline protected eosinophils against IL-5-mediated inhibition of apoptosis with a simultaneous suppression of survival in a dose-dependent manner. Theophylline caused an increase in the intracellular cAMP levels of IL-5-stimulated eosinophils. Enhancement of eosinophil apoptosis was consistent with an increase in DNA fragmentation in eosinophils treated with theophylline. On the other hand, the Bcl-2 protein appeared to be expressed constitutively in freshly isolated eosinophils. Bcl-2 expression was augmented by IL-5 stimulation, yet it was considerably inhibited by theophylline treatment. These data suggest that intracellular cAMP levels and Bcl-2 expression are involved in the suppression of eosinophil survival by theophylline.
- URI
- https://www.sciencedirect.com/science/article/pii/S0008874900916782https://repository.hanyang.ac.kr/handle/20.500.11754/162931
- ISSN
- 0008-8749
- DOI
- 10.1006/cimm.2000.1678
- Appears in Collections:
- COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY[E](과학기술융합대학) > MOLECULAR AND LIFE SCIENCE(분자생명과학과) > Articles
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