Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 진언선 | - |
dc.date.accessioned | 2021-07-06T05:45:59Z | - |
dc.date.available | 2021-07-06T05:45:59Z | - |
dc.date.issued | 2020-03 | - |
dc.identifier.citation | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS, v. 46, article no. 101790 | en_US |
dc.identifier.issn | 2211-9264 | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S2211926419308926?via%3Dihub | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/162680 | - |
dc.description.abstract | Carotenoid pigments have been reported the bioactive substances having an antioxidant effect and anti-cancer effect. Recently, the industrial application of natural violaxanthin with anticancer properties has been expanding. Accordingly, in this study, lutein-deficient Chlorella vulgaris, a high producer of violaxanthin, was generated by chemical random mutagenesis. The lutein-deficient mutant was containing only 15.2% lutein compared to wild type. As a cause of this phenomenon, a single point mutation (A336V) on lycopene epsilon cyclase was identified. Consequently, the competitive metabolic pathway of alpha-branch of xanthophyll synthesis was elevated, causing a 3.18-fold increase in violaxanthin production, compared to that of the wild type. Additionally, the improvement of the growth rate of the mutant (1.3 fold higher than wild type) was observed that caused by enhancement of photosynthesis. As a result of optimization of violaxanthin production, the highest amount of violaxanthin production was achieved under 100 mu mol photons m(-2) s(-1) with acetic acid as the carbon source. Consequently, 3.70 +/- 0.45 mg/g violaxanthin, along with 0.45 +/- 0.03 mg/g and 0.44 +/- 0.02 mg/g of antheraxanthin and zeaxanthin were accumulated in the mutant respectively. These results demonstrate that lutein-deficient Chlorella vulgaris can produce violaxanthin at a concentration equivalent to the highest levels previously reported in marine microalgae and suggesting its potential as a new violaxanthin-producing natural host. | en_US |
dc.description.sponsorship | This research was supported by a grant from the Korea CCS R&D Center (KCRC) (NRF-2014M1A8A1049273), and the Basic Core Technology Development Program for the Oceans and the Polar Regions (NRF-2015M1A5A1037053) of the National Research Foundation of Korea funded by the Korean government (Ministry of Science and ICT). | en_US |
dc.language.iso | en | en_US |
dc.publisher | ELSEVIER | en_US |
dc.subject | Chlorella vulgaris | en_US |
dc.subject | Lutein-deficient | en_US |
dc.subject | Lycopene epsilon cyclase | en_US |
dc.subject | Violaxanthin production | en_US |
dc.title | Development of a Chlorella vulgaris mutant by chemical mutagenesis as a producer for natural violaxanthin | en_US |
dc.type | Article | en_US |
dc.relation.no | 101790 | - |
dc.relation.volume | 46 | - |
dc.identifier.doi | 10.1016/j.algal.2020.101790 | - |
dc.relation.page | 1-7 | - |
dc.relation.journal | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS | - |
dc.contributor.googleauthor | Kim, Jongrae | - |
dc.contributor.googleauthor | Kim, Minjae | - |
dc.contributor.googleauthor | Lee, Suhyeon | - |
dc.contributor.googleauthor | Jin, EonSeon | - |
dc.relation.code | 2020045698 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF NATURAL SCIENCES[S] | - |
dc.sector.department | DEPARTMENT OF LIFE SCIENCE | - |
dc.identifier.pid | esjin | - |
dc.identifier.researcherID | AAW-6839-2020 | - |
dc.identifier.orcid | http://orcid.org/0000-0001-5691-0124 | - |
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