Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이철훈 | - |
dc.date.accessioned | 2021-03-03T02:15:41Z | - |
dc.date.available | 2021-03-03T02:15:41Z | - |
dc.date.issued | 2001-09 | - |
dc.identifier.citation | 산업미생물학회지, v. 29, no. 3, page. 181-185 | en_US |
dc.identifier.issn | 0257-2389 | - |
dc.identifier.uri | http://kiss.kstudy.com/thesis/thesis-view.asp?key=1950321 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/160123 | - |
dc.description.abstract | The naturally occurring chemical indole-3-carbinol (I3C), found in vegetables of the Brassica genus, is a promising anticancer agent that was shown previously to induce a G1 cell cycle arrest of human breast cancer cell lines, independent of estrogen receptor signaling. The anticancer activity of I3C and the possible mechanisms of its action were explored in a human hepatocellular carcinoma cell line, HepG2. Treatment of HepG2 cells with I3C suppressed the growth of the cells. The growth suppression caused by I3C(IC50: 444μM) was found to be partially due to its ability to stop the cell cycle in HepG2 cells. Western blot analysis for the G1 phase arrest demonstrated that the expression-levels of cyclin-dependent kinase (Cdk4, Cdk6) and cyclin D were reduced strongly after treatment of HepG2 cells with I3C (400μM) for 24-72hrs. Furthermore, I3C selectively abolished the expression of Cdk6 in a dose- and time-dependent manner, and accordingly, inhibited the phosphorylation of retinoblastoma. Interestingly, after the HepG2 cells reached their maximal growth arrest, the level of the p21, a well-known Cdk inhibitor, increased significantly. Therefore, it could be considered that the G1 arrest of HepG2 cells treated with I3C was due to the indirect inhibition of Cdk4/6 activities by p21. Western blot analysis for G2/M phase arrest of demonstrated the levels of Cdc2 and cyclin B1 were reduced dramatically after the treatment of HepG2 cells with I3C (40μM) for 24-72hrs. Flow cytometry of propidium iodide-stained HepG2 cells revealed that I3C induces a G1 (53%, 72hr incubation) and G2 (25%, 24hr incubation) cell cycle arrest. Thus, our observations have uncovered a previously undefined antiproliferative pathway for I3C that implicates Cdk4/6 and Cdc2 as a target for cell cycle control in human HepG2 cells. However, the I3C-mediated cell cycle arrest and repression of Cdk4/6 production did not affect the apoptotic induction of HepG2 cell. | en_US |
dc.description.sponsorship | 이 논문은 2000년 한양대학교 교내연구비 지원으로 연구되었음. | en_US |
dc.language.iso | ko_KR | en_US |
dc.publisher | 한국미생물생명공학회(구 한국산업미생물학회) | en_US |
dc.subject | Indole-3-carbinol | en_US |
dc.subject | cell cycle regulation | en_US |
dc.subject | HepG2 cells | en_US |
dc.title | 간암세포주에서의 Indole-3-Carbinol에 의해 유도되는 세포주기 억제 기전 | en_US |
dc.title.alternative | Inhibitory Mechanisms of Cell Cycle Regulation Induced by Indole - 3 - carbinol in Hepatocellular Carcinoma HepG2 Cells | en_US |
dc.type | Article | en_US |
dc.relation.journal | 산업미생물학회지 | - |
dc.contributor.googleauthor | 김동우 | - |
dc.contributor.googleauthor | 이광수 | - |
dc.contributor.googleauthor | 김민경 | - |
dc.contributor.googleauthor | 조율희 | - |
dc.contributor.googleauthor | 이철훈 | - |
dc.relation.code | 2012100712 | - |
dc.sector.campus | E | - |
dc.sector.daehak | COLLEGE OF PHARMACY[E] | - |
dc.sector.department | DEPARTMENT OF PHARMACY | - |
dc.identifier.pid | chhlee | - |
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