Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 류종석 | - |
dc.date.accessioned | 2021-02-17T05:52:58Z | - |
dc.date.available | 2021-02-17T05:52:58Z | - |
dc.date.issued | 2001-03 | - |
dc.identifier.citation | Obstetrics & Gynecology Science, v. 44, no. 3, page. 506-512 | en_US |
dc.identifier.issn | 2287-8572 | - |
dc.identifier.uri | http://kiss.kstudy.com/thesis/thesis-view.asp?key=1857921 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/158647 | - |
dc.description.abstract | Objective : The purpose of this study was to investigate the methods for analysis of restriction fragment length polymorphisms of hemophilia B (coagulation factor Ⅸ) gene in Korean population. Methods : Genomic DNAs were extracted from 40 Korean females. In order to amplify genomic DNAs at the region of the polymorphic sites, two sets of primers (Hha I and Dde I) were synthesized. The primers were named as FIX1, FIX2 for Hha I, and Dde I 59, Dde I 39 for Dde I, respectively. Hha I primers annealed 3'-flanking region of the Factor Ⅸ gene and amplified 230 bp long fragment. The PCR fragment (230 bp) treated with Hha I endonuclease produced two fragments (150 bp and 80 bp), when the polymorphic site existed. Dde I primers annealed the region of the first intron of Factor Ⅸ gene and amplified 319 bp long fragments. People cases with Dde I polymorphic site are supposed to produce 369 bp long fragment. Results : It has been found that seven (14 X chromosomes) out of forty individuals showed Hha I polymorphism. However, none of the experimental People cases showed the Dde I polymorphism. Conclusion : By the analysis of 80 chromosomes, the PICs calculated from allele frequency of Hha I-RFLP (0.175/0.825) and that of Dde I-RFLP (0.0/1.0) were 0.289=[1-(0.1752+0.8252)] and 0=[1-(02+12)], respectively. From these results, it can be postulated that Hha I and Dde I polymorphisms of the Factor Ⅸ gene in Korean exhibited different patterns from those of Caucasian. | en_US |
dc.language.iso | ko_KR | en_US |
dc.publisher | 대한산부인과학회 | en_US |
dc.subject | Blood coagulation factor Ⅸ | en_US |
dc.subject | Hemophilia B | en_US |
dc.subject | RFLP | en_US |
dc.subject | PCR | en_US |
dc.subject | Hha I | en_US |
dc.subject | Dde I | en_US |
dc.title | 한국인 혈액응고 제 9인자의 제한효소 절편 길이 다형성 분석 | en_US |
dc.title.alternative | RFLP Analysis of a Gene for Blood Coagulation Factor Ⅸ in Korean. | en_US |
dc.type | Article | en_US |
dc.relation.no | 3 | - |
dc.relation.volume | 44 | - |
dc.relation.page | 506-512 | - |
dc.relation.journal | 대한산부인과학회지 | - |
dc.contributor.googleauthor | 권무식 | - |
dc.contributor.googleauthor | 이정민 | - |
dc.contributor.googleauthor | 전봉균 | - |
dc.contributor.googleauthor | 오성관 | - |
dc.contributor.googleauthor | 류종석 | - |
dc.contributor.googleauthor | 오보훈 | - |
dc.relation.code | 2012100332 | - |
dc.sector.campus | E | - |
dc.sector.daehak | COLLEGE OF PHARMACY[E] | - |
dc.sector.department | DEPARTMENT OF PHARMACY | - |
dc.identifier.pid | cryou2 | - |
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