The Purification and Characterization of a Bacillus stearothermophilus Methionine Aminopeptidase (MetAP)
- Title
- The Purification and Characterization of a Bacillus stearothermophilus Methionine Aminopeptidase (MetAP)
- Author
- 이영식
- Keywords
- Bacillus stearothermophilus; Methionine aminopeptidase; Co2+ion; Metalloenzyme
- Issue Date
- 2002-10
- Publisher
- 생화학분자생물학회(구 한국생화학분자생물학회)
- Citation
- BMB Reports, v. 35, no. 2, page. 228-235
- Abstract
- Methianine aminopeptidase (MetAP) catalyzes the removal of an amino-terminal methionine from a newly synthesized polypeptide. The enzyme was purified to homogeneity from Bacillus stearothermophilus (KCTC 1752) by a procedure that involves heat precipitation and four sequential chromatographs (including DEAE-Sepharose ion exchange, hydroxylapatite, Ultrogel AcA 54 gel filtration, and Reactive red 120 dye affinity chromatography). The apparent molecular masses of the enzyme were 81,300 Da and 41,000 Da, as determined by gel filtration chromatography and sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE), respectively This indicates that the enzyme is comprised of two identical subunits. The MetAP specifically hydrolyzed the N-terminal residue of Met-Ala-Ser that was used as a substrate, and exhibited a strong preference for Met-Ala-Ser over Leu-Gly-Gly, Leu-Ser-Phe, and Leu-Leu-Tyr. The enzyme has an optimal pH at 8.0, an optimal temperature at 80℃, and pI at 4.1. The enzyme was heat-stable, as its activity remained unaltered when incubated at 80℃ for 45 min. The Km and Vmax values of the enzyme were 3.0 mM and 1.7 mmol/min/㎎, respectively. The B. stearothermophilus MetAP was completely inactivated by EDTA and required Co^(2+) ion(s) for activation, suggesting the metal dependence of this enzyme.
- URI
- http://kiss.kstudy.com/thesis/thesis-view.asp?key=1916437https://repository.hanyang.ac.kr/handle/20.500.11754/157664
- ISSN
- 1976-6696
- Appears in Collections:
- COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY[E](과학기술융합대학) > MOLECULAR AND LIFE SCIENCE(분자생명과학과) > Articles
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