Asiaticoside가 RAW 264.7 세포에서 Inducible nitric oxide synthase 와 Cyclooxygenase-2에 미치는 향염증 작용에 관한 연구

Abstract

Asiaticoside has been tested for the ability as an anti-inflammatory drug using lipopolysaccharide (LPS)-stimulated macrophage cell line (RAW 264.7 cell). LPS treatment induced dramatically inducible nitric oxide synthase (iNOS) in RAW cells. However, asiaticoside inhibited LPSstimulated iNOS induction in a concentration-dependent manner. Especially, higher concentrations (> 50 mM) of asiaticoside completely blocked iNOS induction. In addition, LPS-stimulated expression of inducible cyclooxygenase (COX-2) and interleukin-1a (IL-1a) was inhibited by asiaticoside treatment. Asiaticoside up to 50 mM still required to inhibit COX-2 and IL-1a induced by LPS. Consistent with these findings, treatment with asiaticoside suppressed de novo synthesis and cellular accumulation of prostaglandin E2 to a lesser extent, suggesting that asiaticoside blocked the induction as well as the activity of COX-2. These results suggest the possibility that asiaticoside may be effective therapeutic agents for septic shock and other inflammatory diseases.