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Calnexin and Calreticulin are involved in Chemosensory Behavior in C. elegans

Title
Calnexin and Calreticulin are involved in Chemosensory Behavior in C. elegans
Other Titles
예쁜꼬마선충의 후각신경에 의한 행동조절의 유연성에 관여하는 칼슘결합단백질들에 대한 연구
Author
칼리차미카루나
Alternative Author(s)
Kalichamy Karunambigai
Advisor(s)
안주홍
Issue Date
2009-08
Publisher
한양대학교
Degree
Master
Abstract
Calnexin and calreticulin are calcium binding molecular chaperone proteins in the endoplasmic reticulum (ER). They are involved in the proper assembly of proteins and play pivotal roles during signal transduction. In order to investigate the role of ER calcium binding proteins in experience-dependent behaviors, we studied the C. elegans homologs of both calnexin and calreticulin. Previously we showed that both calnexin and calreticulin are expressed in neurons, and play essential roles during stress conditions. C. elegans is a soil nematode known to detect different volatile odorants using their chemosensory olfactory neurons. Prolonged exposure to an odorant, in wildtype animals, leads to diminished response to the odorant which we term adaptation. Mutants for calnexin (cnx-1) and calreticulin (crt-1) can respond robustly to odorants sensed by the AWC neurons such as isoamyl alcohol, benzaldehyde and butanone. The crt-1 animals exhibit hyperadaptation to AWC sensed odors when compared to wildtype, whereas cnx-1 animals fail to adapt to these odors. These results suggest that CNX-1 may be involved in regulating olfactory adaptation. In addition we quantified the str-2::GFP, a seven transmembrane receptor protein specific in AWC neuronal expression in cnx-1 and crt-1 mutants background. The str-2::GFP expression was abnormal (qualitatively and/or quantitatively) in both cnx-1(nr2009, nr2010) and crt-1(jh101) mutants, suggesting that calcium plays significant roles in receptor expression in AWC neuron. Therefore, we suggest calreticulin and calnexin may regulate adaptation behavior in C. elegans. Currently, we are conducting odorant specific assays and investigating the olfaction mechanism responsible for experience-dependent behaviors involved in calnexin and calreticulin mutants.
URI
https://repository.hanyang.ac.kr/handle/20.500.11754/143999http://hanyang.dcollection.net/common/orgView/200000412037
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > LIFE SCIENCE(생명과학과) > Theses (Master)
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