전기전도도 검출방식의 APTAMER 바이오칩 설계 및 검증에 관한 연구
- Title
- 전기전도도 검출방식의 APTAMER 바이오칩 설계 및 검증에 관한 연구
- Author
- 임태민
- Alternative Author(s)
- Lim, Tae Min
- Advisor(s)
- 안유민
- Issue Date
- 2010-08
- Publisher
- 한양대학교
- Degree
- Master
- Abstract
- Our purpose of this work is a design and fabrication of biosensor for electrochemical detection of thrombin and finding conditions which are aptamer concentration and reaction time, which have a great effect on DNA aptamer immobilization onto gold electrode. In recent reports, microfluidics and electrochemical detection using aptamers which are oligonucleic acid or peptide molecules that bind to a specific target molecule are gradualre concerned because of a simple device to achieve real-time monitoring and fast measurement. In this study, our biosensor cons cos of gold working electrode and pdetinum counter electrode and microchannel based on polydimethylsiloxane (PDMS)-glass. The size of biosensor is in*20 mm2. Gold-sulfur linkage was used as a connection method between working electrode and thrombin binding aptamer(TBA) to specificalre bind for onre gold electrode . It was by fluorescent intensity that we had a final decision of aptamer conditions for immobilization. Our optimal concentration and reaction times are 5nM and 30min, respectively. After then, thrombin detection was performed by cyclic voltammetry. As a results, electrical current was proportional to thrombin concentration. Our biosensor could be used as an effective tool for other molecular diagnostic systems.
- URI
- https://repository.hanyang.ac.kr/handle/20.500.11754/141448http://hanyang.dcollection.net/common/orgView/200000415372
- Appears in Collections:
- GRADUATE SCHOOL[S](대학원) > MECHANICAL ENGINEERING(기계공학과) > Theses (Master)
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