골드 전극 위에 고정화한 TLR4/MD-2 복합체를 이용한 전기화학적 엔도톡신 검출 시스템

Abstract

Over the past decades, sepsis and septic shock, caused by bacterial infection, are one of the main causes of death in intensive care units and a significant threat on public health. Endotoxin is a component of cell wall in the outer membrane of gram-negative bacteria. It is an inducer of the innate immune response and a low concentration of endotoxin causes fatal symptoms of shock. Currently, the limulus amebocyte lysate (LAL) method is most widely used as an endotoxin detection system but they have some drawbacks that are low accuracy, subjective recognition of clot, long detection time and low sensitivity. For these reasons, various detection methods and recognition substances have been suggested to overcome these problems such as low sensitivity, inconvenience, and long detection time. Generally, TLR4 in association with MD-2 was known to play a role in the innate immune response and to bind to the endotoxin specifically. Due to the high affinity between TLR4/MD-2 complex and endotoxin, the rhTLR4/MD-2 complex was used as a detection substance of endotoxin. An electrochemical analysis was employed as an endotoxin detection method and it was conducted by cyclic voltametry (CV) and differential pulse voltametry (DPV). In this study, a new endotoxin detection system was developed successfully, based on electrochemical detection systems using rhTLR4/MD-2 complexe immobilized on the gold electrode.