세포 고정 분자의 잉크젯 프린팅을 통한 세포칩 제작

Abstract

Well-defined cell patterns, including both adherent and nonadherent cells, were created through the piezoelectric inkjet printing of a cell membrane-anchoring polymer, which is composed of a hydrophobic oleyl group, a hydrophilic poly(ethylene glycol) (PEG) chain, and an amino-reactive N-hydroxy-succinimide (NHS) end group. Various complex living cell patterns were created by surface engineering in which inkjet-printed, membrane-anchoring polymer patterns controlled the geometric distributions of immobilized cells. Non-adherent cells (SNU-620) as well as adherent cells (HeLa) were stably anchored and patterned on a glass substrate through interactions between cell membranes and the hydrophobic oleyl groups. The effects of an apoptosis inducer (staurosporine) and an anticancer drug (paclitaxel) were visualized on cells immobilized on the polymer-printed patterns. This approach may have broad utility in most advanced biomedical devices requiring miniaturized, precisely controlled, living cell patterns.