Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이철훈 | - |
dc.date.accessioned | 2020-03-09T06:13:50Z | - |
dc.date.available | 2020-03-09T06:13:50Z | - |
dc.date.issued | 2004-03 | - |
dc.identifier.citation | 한국미생물·생명공학회지, v. 32, No. 1, Page. 72-77 | en_US |
dc.identifier.issn | 1598-642x | - |
dc.identifier.uri | http://kiss.kstudy.com/thesis/thesis-view.asp?key=2215861 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/134677 | - |
dc.description.abstract | A Cyclin-Dependent Kinase Inhibitor, p16INK4A, Induces Apoptosis in The Human Cancer Cells. Kim, Min-Kyoung and Chul-Hoon Lee*. Department of Medical Genetics, Hanyang University College of Medicine, Seoul 133-791, Korea - Previously, we synthesized a novel Cyclin-dependent kinase inhibitor, MCS-5A. Also, we investigated the involvement of cell cycle regulatory events during MCS-5A-mediated apoptosis in HL-60(+p16/-p53) cells with up-regulation of p16 protein expression. In contrast, apoptosis was not observed in A549(-p16/+p53) cells. Therefore we propose that p16INK4A is a key enzyme for inducing apoptosis. In the present studies, we have explored the mechanism of p16INK4A-mediated cytotoxicity and the role of p16INK4A overexpression in the induction of apoptosis in human tumor cells. The tumor suppressor gene p16INK4A is known as a cyclin-dependent kinase inhibitor (CKI) and cell cycle regulator. We expressed wild type p16INK4A in pcDNA3.1 vector and then transfected into non-small cell lung cancer (NSCLC) cell expressing different statue of p16INK4A, p53 gene[A549(-p16/+p53), H1299(-p16/-p53) and HeLa (+p16/+p53)cell line]. TUNEL assay (including propidium iodide staining following transfection of these cell line with pcDNA3.1-p16) indicate that p16INK4A-mediated cytotoxicity was associated with apoptosis. This is supported by studies demonstrating an induction of caspase 3 cleavage due to the transfection of A549, H1299 and HeLa cells with pcDNA3.1-p16. These results suggest that p16INK4A has a new function of inducing apoptosis which is not related with the function of tumor suppressor gene p.53 | en_US |
dc.description.sponsorship | 이 논문은 2001년 한양대학교 교내연구비 지원으로 연구 되었음. | en_US |
dc.language.iso | ko_KR | en_US |
dc.publisher | 한국미생물생명공학회 | en_US |
dc.subject | P16(INK4A) | en_US |
dc.subject | Apoptosis | en_US |
dc.subject | Cell cycle arrest | en_US |
dc.title | Cyclin-dependent kinase 저해 단백질 p16INK4A의 인체 암세포에서의 세포사멸 유도 활성 | en_US |
dc.title.alternative | A Cyclin-Dependent Kinase Inhibitor, pl6(INK4A), Induces Apoptosis in The Human Cancer Cells | en_US |
dc.type | Article | en_US |
dc.relation.journal | 한국미생물·생명공학회지 | - |
dc.contributor.googleauthor | 김민경 | - |
dc.contributor.googleauthor | 이철훈 | - |
dc.relation.code | 2012211005 | - |
dc.sector.campus | E | - |
dc.sector.daehak | COLLEGE OF PHARMACY[E] | - |
dc.sector.department | DEPARTMENT OF PHARMACY | - |
dc.identifier.pid | chhlee | - |
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