The Development of Novel Cyanine Dyes with Vinylsulfone Group for the Optical Imaging
- Title
- The Development of Novel Cyanine Dyes with Vinylsulfone Group for the Optical Imaging
- Other Titles
- 광학 영상을 위한 신규의 비닐설폰계 시아닌 염료의 개발
- Author
- 박진우
- Alternative Author(s)
- Park, Jin Woo
- Advisor(s)
- 이기정
- Issue Date
- 2013-02
- Publisher
- 한양대학교
- Degree
- Doctor
- Abstract
- In this thesis, novel vinylsulfone (VS) cyanine dyes (em. 550−850 nm) were designed and synthesized for fluorescent labeling of biomolecules via 1,2-addition reaction in aqueous conditions. Due to the virtue of chemical structures of both fluorophore and reactive group, these dyes could be significantly stable and reactive in various aqueous/organic conditions. A wide variety of pH, temperature, buffer concentration, and protein were tested for the optimal labeling condition.
Also, novel cyanine dyes with a VS group were designed and synthesized for pH indication of biomolecules. Among the series of pH indicator candidates, a selected reactive dye and its labeled biomolecules showed a dramatic change of fluorescence intensity in a wide range of pH conditions. The pH indicating dye can be used to label protein molecules and maintains its optical properties when conjugated to a macromolecule, making it possible to observe pH distributions and changes in acidic and neutral pH conditions.
Finally, as an application study using the VS cyanine dyes, we developed a wide ranged fluorescent molecular weight size marker (MWSM). These MWSMs covers upto near-infrared wavelengths, consisted of sequentially arranged VS cyanine dyes enabling proteomic analysis without post-stain for size indication.
- URI
- https://repository.hanyang.ac.kr/handle/20.500.11754/133595http://hanyang.dcollection.net/common/orgView/200000421019
- Appears in Collections:
- GRADUATE SCHOOL[S](대학원) > CHEMICAL ENGINEERING(화학공학과) > Theses (Ph.D.)
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