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Basal-type lumenogenesis in extraembryonic endoderm stem cells models the early visceral endoderm

Title
Basal-type lumenogenesis in extraembryonic endoderm stem cells models the early visceral endoderm
Author
Binas, Bert
Keywords
Stem cells; Visceral endoderm; Vacuole; Lumen; Vesicle; Polarity
Issue Date
2019-10
Publisher
COMPANY BIOLOGISTS LTD
Citation
JOURNAL OF CELL SCIENCE, v. 132, No. 19, Article no. UNSP jcs230607
Abstract
Cultured rat primitive extraembryonic endoderm (pXEN) cells easily form free-floating multicellular vesicles de novo, exemplifying a poorly studied type of morphogenesis. Here, we reveal the underlying mechanism and the identity of the vesicles. We resolve the morphogenesis into vacuolization, vesiculation and maturation, and define the molecular characteristics and requirements of each step. Vacuolization is fueled by macropinocytosis and occurs by default if not blocked by high cell density or matrix proteins. Fine-tuned cell-cell contact then forms nascent three-cell vesicles with vacuolederived lumina. In maturation, the vesicles complete epithelialization, expand viamitosis and continued fluid uptake, and differentiate further. The mature vesicles consist of a simple squamous epithelium with an apical-outside/basal-inside polarity that we trace back to the single cell stage. The polarity and gene expression pattern of the vesicles are similar to those of the early visceral endoderm. pXEN cells provide a useful in vitro model for study of matrix-independent, basal-type lumenogenesis and the physiology of the visceral endoderm.
URI
https://jcs.biologists.org/content/132/19/jcs230607.abstracthttp://repository.hanyang.ac.kr/handle/20.500.11754/121269
ISSN
0021-9533; 1477-9137
DOI
10.1242/jcs.230607
Appears in Collections:
COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY[E](과학기술융합대학) > MOLECULAR AND LIFE SCIENCE(분자생명과학과) > Articles
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