Development of quantum dot aptasensor and its portable analyzer for the detection of di-2-ethylhexyl phthalate
- Title
- Development of quantum dot aptasensor and its portable analyzer for the detection of di-2-ethylhexyl phthalate
- Author
- 윤문영
- Keywords
- Quantum dot aptasensor; Di-2-ethylhexyl phthalate; Aptamer; Portable analyzer
- Issue Date
- 2018-12
- Publisher
- ELSEVIER ADVANCED TECHNOLOGY
- Citation
- BIOSENSORS & BIOELECTRONICS, v. 121, page. 1-9
- Abstract
- We have developed a quantum dot aptasensor (QD-aptasensor) and its accompanying portable analyzer for the detection of di-2-ethylhexyl phthalate (DEHP). This sensor is based on a newly screened aptamer (60-mer) via SELEX and shows a binding affinity of 213 nmol/L with DEHP. The 60-mer aptamer together with its three shorter truncated aptamers (45, 28, and 22-mer) as well as three different DNA probes (12, 9, and 13-mer) were further investigated to form the best combination for the QD-aptasensor. Using a 22-mer-truncated aptamer and a 12-mer DNA probe combination, the QD-aptasensor demonstrated excellent DEHP sensitivity with an LOQ = 0.5 pg/mL as well as good selectivity in the presence of other phthalate analogs. The binding between the truncated aptamers and DEHP was also characterized. Finally, a QD-aptasensor-based portable analyzer was also developed, and its equivalence to the laboratory protocol was established with a correlation coefficient r = 0.86 for DEHP concentrations ranging from 0.0005 to 100 ng/mL.
- URI
- https://www.sciencedirect.com/science/article/pii/S0956566318306766?via%3Dihubhttps://repository.hanyang.ac.kr/handle/20.500.11754/120992
- ISSN
- 0956-5663; 1873-4235
- DOI
- 10.1016/j.bios.2018.08.065
- Appears in Collections:
- COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > CHEMISTRY(화학과) > Articles
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